Antipov A N, Lyalikova N N, Khijniak T V, L'vov N P
A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow.
IUBMB Life. 2000 Jul;50(1):39-42. doi: 10.1080/15216540050176575.
Molybdenum- and molybdenum cofactor-free nitrate reductases recently isolated by us from vanadate-reducing bacteria Pseudomonas isachenkovii are likely to mediate vanadate reduction. During anaerobic growth of P. isachenkovii on medium supplemented with nitrate and vanadate, vanadate dissimilation was followed by nitrate consumption, and this process was associated with some structural reorganizations of nitrate reductases. The homogeneous membrane-bound nitrate reductase of P. isachenkovii reduced vanadate with NADH as an electron donor.
我们最近从能还原钒酸盐的细菌伊萨琴科夫假单胞菌中分离出的无钼和无钼辅因子的硝酸还原酶可能介导钒酸盐的还原。在伊萨琴科夫假单胞菌在添加了硝酸盐和钒酸盐的培养基上厌氧生长期间,钒酸盐异化之后是硝酸盐消耗,并且这个过程与硝酸还原酶的一些结构重组有关。伊萨琴科夫假单胞菌的均一膜结合硝酸还原酶以NADH作为电子供体还原钒酸盐。