采用高温高分辨气相色谱-质谱联用技术快速筛查巴西蜂胶中的极性化合物。

Rapid screening of polar compounds in Brazilian propolis by high-temperature high-resolution gas chromatography-mass spectrometry.

作者信息

Pereira A S, Norsell M, Cardoso J N, Aquino Neto F R, Ramos M F

机构信息

Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, CCS, Bloco K, 2 degrees Andar, Rio de Janeiro, RJ-Brazil 21941-590.

出版信息

J Agric Food Chem. 2000 Nov;48(11):5226-30. doi: 10.1021/jf000594p.

DOI:10.1021/jf000594p

PMID:11087464

Abstract

Methanol extracts of propolis from six different places, five in Rio de Janeiro state and one in São Paulo state, both in the Southeast of Brazil, were investigated using high-temperature high-resolution gas chromatography (HT-HRGC) and HT-HRGC-mass spectometry. The main purpose of the study was to establish the applicability of HT-HRGC as an analytical method for systematic studies of polar propolis fractions. Several compounds, including carbohydrates, phenolic acid derivatives, and high molecular weight compounds (e.g., wax esters of long chain fatty alcohols) could be readily characterized in the crude extracts by HT-HRGC-MS. HT-HRGC and HT-HRGC-MS were shown to be quick and informative tools for rapid analysis of crude polar extracts without cleanup.

摘要

对来自巴西东南部里约热内卢州的5个地方以及圣保罗州的1个地方的6种不同蜂胶的甲醇提取物，采用高温高分辨气相色谱法（HT-HRGC）和高温高分辨气相色谱-质谱联用法进行了研究。该研究的主要目的是确定HT-HRGC作为一种分析方法用于极性蜂胶馏分系统研究的适用性。通过HT-HRGC-MS可轻松对粗提物中的几种化合物进行表征，包括碳水化合物、酚酸衍生物和高分子量化合物（如长链脂肪醇的蜡酯）。结果表明，HT-HRGC和HT-HRGC-MS是无需净化即可快速分析极性粗提物的快速且信息丰富的工具。