大肠杆菌嘧啶饥饿期间核糖体蛋白合成的调控
Regulation of synthesis of ribosomal proteins during pyrimidine starvation in Escherichia coli.
作者信息
Beatty B G, Mak W W, Wong J T
出版信息
Biochem J. 1975 Sep;150(3):463-8. doi: 10.1042/bj1500463.
Abstract
The synthesis of ribosomal proteins during pyrimidine starvation was investigated. A progressive turn-off of protein synthesis, with a decay half-time of about 5 min, was observed when Escherichia coli cells were starved of uridine. By means of double-labelling, the syntheses of different ribosomal proteins were shown to be turned off unequally during the starvation. Comparison of the turn-off patterns for some proteins and the known polycistronic organization of the structural genes for these proteins suggests that a major cause of the unequal turn-off was the degradation of mRNA molecules for the ribosomal proteins from the 5'-end toward the 3'-end.
摘要
对嘧啶饥饿期间核糖体蛋白的合成进行了研究。当大肠杆菌细胞缺乏尿苷时，观察到蛋白质合成逐渐停止，衰减半衰期约为5分钟。通过双重标记表明，在饥饿期间不同核糖体蛋白的合成停止情况并不相同。比较一些蛋白质的停止模式以及这些蛋白质结构基因已知的多顺反子组织，表明这种不平等停止的主要原因是核糖体蛋白的mRNA分子从5'端向3'端降解。
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