Jones C S, Davies H V, Taylor M A
Unit of Plant Biochemistry, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Planta. 2000 Oct;211(5):708-14. doi: 10.1007/s004250000336.
Processes that contribute to the overall phenomenon of fruit ripening are not well understood for many soft fruit species, including raspberry (Rubus idaeus L.) Recent biochemical data implicate ethylene and a range of cell wall hydrolases in ripening processes. However, the genes encoding these activities, and others related to ripening, remain to be characterised. With the advent of high-throughput RNA-fingerprinting techniques it is possible to characterise rapidly the changes in gene expression during developmental processes. This paper describes the application of two RNA-fingerprinting techniques (cDNA amplified fragment length polymorphism and differential display reverse transcription-polymerase chain reaction) to the ripening fruit of Rubus idaeus. Copy-DNA tags were isolated representing 34 genes, up-regulated during fruit ripening. The expression profiles of these genes were determined by RNA-blot analysis and their sequences were compared with those in public databases. Potential roles for some of these gene products are considered, providing valuable insights into the processes that underpin fruit ripening. Many of the cDNAs isolated in this study provide tools for the biotechnological improvement of fruit quality.
对于包括树莓（Rubus idaeus L.）在内的许多软果品种，导致果实成熟这一整体现象的过程尚未得到充分了解。最近的生化数据表明乙烯和一系列细胞壁水解酶参与了成熟过程。然而，编码这些活性以及其他与成熟相关的基因仍有待鉴定。随着高通量RNA指纹技术的出现，能够快速鉴定发育过程中基因表达的变化。本文描述了两种RNA指纹技术（cDNA扩增片段长度多态性和差异显示逆转录-聚合酶链反应）在树莓成熟果实中的应用。分离出了代表34个在果实成熟过程中上调的基因的cDNA标签。通过RNA印迹分析确定了这些基因的表达谱，并将它们的序列与公共数据库中的序列进行了比较。考虑了其中一些基因产物的潜在作用，为深入了解果实成熟的基础过程提供了有价值的见解。本研究中分离出的许多cDNA为果实品质的生物技术改良提供了工具。
