Wullimann M F, Knipp S
Brain Research Institute, University of Bremen, Germany.
Anat Embryol (Berl). 2000 Nov;202(5):385-400. doi: 10.1007/s004290000115.
Proliferating cell nuclear antigen (PCNA)-immunohistochemistry was used for demonstrating the spatiotemporal course of proliferation in the brains of embryonic (24 h) through postembryonic (5 days) zebrafish (Danio rerio). Parallel series of the same stages prepared according to the combined Bodian fiber silver-stain/cresyl Nissl-stain were used for improved morphogenetic analysis (i.e., in detecting critical neuroanatomical landmarks). Starting from an essentially ubiquituous proliferation throughout the neural tube before 24 h, PCNA-immunoreactive cells become successively more restricted to a subset of gray matter cells around 48 h and even more distinct proliferation zones become apparent around 72 h. Both hindbrain and forebrain reveal a segmental organization with regard to the distribution of proliferation zones, but the rhombomeric pattern of PCNA-immunoreactive cells emerging between 48 h and 72 h precedes a similar prosomeric pattern by about 48 h. Two divisions of the midbrain-hindbrain boundary are described here morphologically and both are demonstrated to show sustained proliferation throughout the investigated time frame. In contrast, proliferation in the adjacent mesencephalic and cerebellar domains is rapidly down-regulated during the first 5 days of development.
增殖细胞核抗原（PCNA）免疫组织化学被用于展示斑马鱼（Danio rerio）从胚胎期（24小时）到胚后期（5天）大脑中增殖的时空过程。根据改良的博迪安纤维银染/甲酚紫尼氏染色法制备的相同阶段的平行系列样本用于改进形态发生分析（即检测关键的神经解剖学标志）。在24小时之前，增殖细胞核抗原免疫反应性细胞在整个神经管中基本普遍存在，到48小时左右，这些细胞逐渐更局限于灰质细胞的一个子集，到72小时左右，更明显的增殖区变得明显。后脑和前脑在增殖区的分布方面都显示出节段性组织，但在48小时至72小时之间出现的增殖细胞核抗原免疫反应性细胞的菱脑节模式比类似的前脑节模式提前约48小时出现。本文从形态学上描述了中脑 - 后脑边界的两个分区，并且在整个研究时间范围内都显示出持续增殖。相比之下，在发育的前5天，相邻的中脑和小脑区域的增殖迅速下调。
