Wullimann M F, Puelles L
Brain Research Institute, University of Bremen, Germany.
Anat Embryol (Berl). 1999 Apr;199(4):329-48. doi: 10.1007/s004290050232.
Large gaps of knowledge exist regarding postembryonic brain morphogenesis of the zebrafish Danio rerio (Cyprinidae, Teleostei). The zebrafish represents together with the frog (Xenopus), chick and mouse--one of four major models for the genetic study of early brain development. Here, we used normal silver-stained Bodian material and immunohistochemical material stained with a monoclonal antibody against the proliferating cell nuclear antigen (PCNA, cyclin) to study the morphogenetic appearance and location of proliferation zones of the zebrafish brain between day 1 and day 10, focussing on the forebrain at day 5 postfertilization. Our results directly demonstrate that the dorsal telencephalic proliferation zone (i.e. the pallium) extends--consistent with the process of eversion--some distance laterally on top of the telencephalon. The subpallial telencephalic proliferation consists of dorsal and ventral zones. The preoptic region also includes dorsal and ventral proliferation zones. In the diencephalon proper, separate proliferation zones are present in the habenula, and in the periventricular cell masses of the dorsal thalamus, the ventral thalamus, and the pretectum. More ventrocaudally, the latter three massive proliferation zones appear to be replaced each by thinner, but distinct proliferation zones. Two of them represent ventrocaudal continuations of the dorsal and ventral thalamus and lie in the region referred to as the posterior tubercular area in adult teleostean neuroanatomy. The third lies in the region of the nucleus of the medial longitudinal fascicle. In addition, several hypothalamic proliferation zones are present. The data for the diencephalon are largely in agreement with the neuromeric model of brain organization of Puelles and Rubenstein (1993), which is mostly based on amniote data. Generally, the understanding of the prosomeric origin of teleostean prosencephalic cell masses may be regarded as pivotal for their comparative interpretation.
关于斑马鱼（鲤科，硬骨鱼纲）胚后脑部形态发生，目前存在大量知识空白。斑马鱼与青蛙（非洲爪蟾）、鸡和小鼠一同，是早期脑发育基因研究的四大主要模型之一。在此，我们使用正常的博迪安银染材料以及用抗增殖细胞核抗原（PCNA，细胞周期蛋白）单克隆抗体染色的免疫组织化学材料，研究了斑马鱼在1日龄至10日龄之间脑部增殖区的形态发生外观和位置，重点关注受精后第5天的前脑。我们的结果直接表明，背侧端脑增殖区（即大脑皮质）随着外翻过程，在端脑顶部横向延伸了一段距离。端脑皮质下增殖区由背侧和腹侧区域组成。视前区也包括背侧和腹侧增殖区。在间脑本身，缰核以及背侧丘脑、腹侧丘脑和顶盖前区的室周细胞团中存在独立的增殖区。在更腹尾侧，后三个大量增殖区似乎分别被更薄但明显的增殖区所取代。其中两个代表背侧和腹侧丘脑的腹尾延续，位于成年硬骨鱼神经解剖学中称为后结节区的区域。第三个位于内侧纵束核所在区域。此外，还存在几个下丘脑增殖区。间脑的数据在很大程度上与普列尔斯和鲁宾斯坦（1993年）的脑组织结构神经节段模型一致，该模型主要基于羊膜动物数据。一般来说，对硬骨鱼前脑细胞团的原节起源的理解可能被视为对其进行比较解释的关键。
