van der Werf M J, Keijzer P M, van der Schaft P H
Division of Industrial Microbiology, Department of Food Technology and Nutritional Sciences, Wageningen University, Wageningen, The Netherlands.
J Biotechnol. 2001 Nov 30;84(2):133-43. doi: 10.1016/s0168-1656(00)00348-5.
Xanthobacter sp. C20 was isolated from sediment of the river Rhine using cyclohexane as sole source of carbon and energy. Xanthobacter sp. C20 converted both enantiomers of limonene quantitatively into limonene-8,9-epoxide, a not previously described bioconversion product of limonene. With (4R)-limonene, (4R,8R)-limonene-8, 9-epoxide was formed as the only reaction product, while (4S)-limonene was converted into a (78:22) mixture of (4S,8R)- and (4S,8S)-limonene-8,9-epoxide. Cytochrome P-450 was shown to be induced concomitantly with limonene bioconversion activity following growth of Xanthobacter sp. C20 on cyclohexane. Maximal limonene bioconversion rate was observed at an initial substrate concentration of 12 mM. The amount of limonene-8,9-epoxide formed, up to 0.8 g l(-1), was limited by a strong product inhibition.
从莱茵河沉积物中分离出以环己烷作为唯一碳源和能源的黄色杆菌属菌株C20。黄色杆菌属菌株C20将苎烯的两种对映体都定量转化为苎烯-8,9-环氧化物，这是一种之前未描述过的苎烯生物转化产物。对于(4R)-苎烯，仅形成(4R,8R)-苎烯-8,9-环氧化物作为唯一反应产物，而(4S)-苎烯则转化为(4S,8R)-和(4S,8S)-苎烯-8,9-环氧化物的(78:22)混合物。在黄色杆菌属菌株C20在环己烷上生长后，细胞色素P-450被证明与苎烯生物转化活性同时被诱导。在初始底物浓度为12 mM时观察到最大苎烯生物转化率。高达0.8 g l(-1)的苎烯-8,9-环氧化物形成量受到强烈的产物抑制作用限制。
