Pisarev V M, Parajuli P, Mosley R L, Sublet J, Kelsey L, Sarin P S, Zimmerman D H, Winship M D, Talmadge J E
Laboratory of Transplantation Immunology, Department of Pathology/Microbiology, 985660 Nebraska Medical Center, Omaha, NE 68198-5660, USA.
Int J Immunopharmacol. 2000 Nov;22(11):865-76. doi: 10.1016/s0192-0561(00)00048-5.
Liposomes and Flt3 ligand (Flt3L), a ligand for the fms-like tyrosine kinase receptor Flt3/ FLK2, can augment the immune response to an HIV peptide vaccine. The HGP-30 peptide used in these studies is a synthetic peptide that corresponds to a highly conserved region of HIV-1 p17 gag (amino acids 86-115). Mice were immunized with HGP-30 or HGP-30 conjugated to keyhole limpet hemocyanin (KLH) and delayed-type hypersensitivity (DTH) responses, antibody (IgG) amount and antigen-specific proliferative responses by spleen cells were used to monitor the immune response. Daily injections of Flt3L prior to HGP-30 administration enhanced significantly an antigen-specific lymphocyte proliferation response when compared with Flt3L, HGP-30 alone or HGP-30 containing liposomes. Intravenous administration of HGP-30 was superior to intramuscular (i.m.) immunization for the induction of DTH responses. The HGP-30/KLH containing liposomes enhanced both DTH and antibody responses, while liposomes containing HGP-30 peptide elicited only T cell responses. In these studies, either Flt3L or liposomes increased DTH responses compared with the i.m. injection of the HGP-30 vaccine alone.
脂质体和Flt3配体（Flt3L），一种类fms酪氨酸激酶受体Flt3/FLK2的配体，可增强对HIV肽疫苗的免疫反应。这些研究中使用的HGP - 30肽是一种合成肽，对应于HIV - 1 p17 gag的高度保守区域（氨基酸86 - 115）。用HGP - 30或与钥孔血蓝蛋白（KLH）偶联的HGP - 30免疫小鼠，并通过脾细胞的迟发型超敏反应（DTH）、抗体（IgG）量和抗原特异性增殖反应来监测免疫反应。与单独使用Flt3L、HGP - 30或含HGP - 30的脂质体相比，在给予HGP - 30之前每日注射Flt3L可显著增强抗原特异性淋巴细胞增殖反应。静脉注射HGP - 30在诱导DTH反应方面优于肌肉注射（i.m.）免疫。含HGP - 30/KLH的脂质体增强了DTH和抗体反应，而含HGP - 30肽的脂质体仅引发T细胞反应。在这些研究中，与单独肌肉注射HGP - 30疫苗相比，Flt3L或脂质体均可增强DTH反应。
