Finan R R, Irani-Hakime N, Tamim H, Almawi W Y
Department of Obstetrics and Gynecology, St. Georges-Orthodox Hospital, P.O. Box 166378-6417, Beirut, Lebanon.
J Clin Virol. 2000 Dec;19(3):163-8. doi: 10.1016/s1386-6532(00)00097-4.
The aim of this study was to assess the validity and usefulness of vaginal scrapes in detecting cervical human papillomavirus (HPV) DNA by the polymerase chain reaction (PCR). The study group comprised 23 women tested positive and 28 women tested negative for cervical HPV DNA by PCR, and confirmed by histopathology. At the time of specimen collection, both vaginal and endocervical scrapes were taken from these women, and tested for HPV DNA by PCR, using MY09/MY11 primer system. HPV genotypes were analyzed by hybridizing PCR products with HPV type-specific biotinylated probes. HPV DNA was detected in both vaginal and cervical scrapes from the HPV-positive, but not from HPV-negative group. In the HPV-positive group, the same HPV type was found in vaginal and endocervical scrapes, giving a positive predictive value of 1.0. The results indicate that HPV types can be detected in vaginal scrapes, and recommend utilization of the less invasive vaginal testing for the routine detection of HPV DNA.
本研究的目的是评估阴道刮片通过聚合酶链反应（PCR）检测宫颈人乳头瘤病毒（HPV）DNA的有效性和实用性。研究组包括23名经PCR检测宫颈HPV DNA呈阳性且经组织病理学证实的女性，以及28名经PCR检测宫颈HPV DNA呈阴性的女性。在采集标本时，从这些女性身上同时采集阴道和宫颈刮片，并使用MY09/MY11引物系统通过PCR检测HPV DNA。通过将PCR产物与HPV型特异性生物素化探针杂交来分析HPV基因型。在HPV阳性组的阴道和宫颈刮片中均检测到HPV DNA，而在HPV阴性组中未检测到。在HPV阳性组中，阴道和宫颈刮片中发现相同的HPV型，阳性预测值为1.0。结果表明，可在阴道刮片中检测到HPV型，并建议采用侵入性较小的阴道检测方法用于HPV DNA的常规检测。
