Quintanilla M E, Tampier L
Program of Molecular and Clinical Pharmacology ICBM, Faculty of Medicine, University of Chile, PO Box 70.000, 7, Santiago, Chile.
Alcohol. 2000 Jul;21(3):245-9. doi: 10.1016/s0741-8329(00)00092-6.
The effect of naltrexone (a non-selective opioid receptor antagonist) on both alcohol consumption in a voluntary selection situation and acute tolerance to the motor impairment effect of ethanol was examined in female high alcohol-drinking (UChB) rats using the tilting plane test. In experiment 1, the effect of naltrexone on alcohol consumption was studied in UChB rats which were given a daily 1-h period access to a 10% (v/v) ethanol solution with food and water always available. Naltrexone in doses of 5 or 10 mg/kg intaperitoneal (i.p.) caused dose-dependent reduction in voluntary alcohol intake by 45% and 66%, respectively, without altering daily water intake. In experiment 2, the effect of naltrexone (5 mg/kg i.p.) on acute tolerance to motor impairment effect of a dose of 2.3 g ethanol/kg i.p. was examined. A comparison of control (C) and naltrexone (Nal) UChB groups indicated that naltrexone slowed the recovery of the motor activity and reduce acute tolerance development at comparable ethanol levels in cerebral blood. These results suggest a contribution of the opioid system to acute tolerance to ethanol.
使用倾斜平面试验，在高饮酒量的雌性大鼠（UChB大鼠）中研究了纳曲酮（一种非选择性阿片受体拮抗剂）对自愿选择情况下酒精摄入量以及对乙醇运动损伤效应的急性耐受性的影响。在实验1中，对UChB大鼠给予每天1小时的时间饮用10%（v/v）乙醇溶液，同时食物和水始终供应，研究纳曲酮对酒精摄入量的影响。腹腔注射（i.p.）5或10mg/kg剂量的纳曲酮分别使自愿酒精摄入量呈剂量依赖性降低45%和66%，而不改变每日水摄入量。在实验2中，研究了纳曲酮（5mg/kg i.p.）对腹腔注射2.3g乙醇/kg剂量的运动损伤效应的急性耐受性的影响。对照（C）组和纳曲酮（Nal）组UChB大鼠的比较表明，在脑血流量中乙醇水平相当的情况下，纳曲酮减缓了运动活动的恢复并降低了急性耐受性的发展。这些结果表明阿片系统对乙醇急性耐受性有影响。
