Thaëron C, Avaron F, Casane D, Borday V, Thisse B, Thisse C, Boulekbache H, Laurenti P
Laboratoire de Biologie du Développement, EA 296, Université de Paris 7, case courrier 7077, 2 place Jussieu, 75251 cedex 5, Paris, France.
Mech Dev. 2000 Dec;99(1-2):167-72. doi: 10.1016/s0925-4773(00)00473-1.
The even-skipped-related homeobox genes (evx) are widely distributed through animal kingdom and are thought to play key role in posterior body patterning and neurogenesis. We have cloned and analyzed the expression of evx1 in zebrafish (see also Borday et al. (Dev. Dyn. 220 (2001) in press) which displays a dynamic and restricted expression pattern during neurogenesis. In spinal cord, rhombencephalon, and epiphysis, evx1 is expressed in several subsets of emerging interneurones prior to their axonal outgrowth, identified as primary interneurones and a subset of Pax2.1(+) commissural interneurones. In the hindbrain, evx1 is expressed in reticulospinal interneurones of rhombomeres 5 and 6 as well as in rhombomere 7 interneurones. The latest emerging evx1(+) interneurones in the hindbrain correspond to commissural interneurones. evx1 is also dynamically transcribed during the formation of the posterior gut and the uro-genital system in mesenchymal cells that border the pronephric ducts, the wall of the pronephric duct, and later in the posterior gut and the wall of the uro-genital opening. In larvae, the ano-rectal epithelium and the muscular layer that surrounds the analia-genitalia region remain stained up to 27 days. In contrast other vertebrates, evx1displays no early nor caudal expression in zebrafish.
偶数跳格相关同源框基因（evx）广泛分布于动物界，被认为在身体后部模式形成和神经发生中起关键作用。我们克隆并分析了斑马鱼中evx1的表达（另见Borday等人，《发育动力学》220卷（2001年）待发表），其在神经发生过程中呈现动态且受限的表达模式。在脊髓、菱脑和松果体中，evx1在轴突长出之前在一些新出现的中间神经元亚群中表达，这些亚群被确定为初级中间神经元和一部分Pax2.1(+)连合中间神经元。在后脑中，evx1在菱节5和6的网状脊髓中间神经元以及菱节7的中间神经元中表达。后脑中最晚出现的evx1(+)中间神经元对应于连合中间神经元。在紧邻原肾管、原肾管壁的间充质细胞中，以及随后在后肠和泌尿生殖孔壁中，evx1在中肠和泌尿生殖系统形成过程中也进行动态转录。在幼虫中，肛门直肠上皮和围绕肛门生殖器区域的肌肉层在27天内一直保持染色。与其他脊椎动物不同，evx1在斑马鱼中没有早期或尾部表达。
