Icatlo F C, Yokoyama H, Kuroki M, Kobayashi C, Goshima H, Ikemori Y, Kodama Y
Immunology Research Institute, Ghen Corporation, Gifu, Japan.
Microbiol Immunol. 2000;44(9):773-6. doi: 10.1111/j.1348-0421.2000.tb02562.x.
Colonization by Helicobacter pylori partly depends on acid-dependent adherence by urease to gastric mucin. To further verify the relevance of urease adherence to colonization, the influence of acidity on the binding sites of H. pylori urease was investigated. When enzyme-based in vitro ligand capture assays were used, the effect of acidity on the binding site of H. pylori urease was determined against a backdrop medium consisting of acidic buffers simulating the luminal side of gastric mucus. A high degree of stability was exhibited by adherent urease, suggesting a pivotal role by the denatured enzyme in the persistence of the bacterium within the acidified compartment of gastric mucus.
幽门螺杆菌的定殖部分取决于脲酶通过酸依赖性方式黏附于胃黏液。为进一步验证脲酶黏附与定殖的相关性，研究了酸度对幽门螺杆菌脲酶结合位点的影响。当使用基于酶的体外配体捕获分析时，在由模拟胃黏液腔侧酸性缓冲液组成的背景介质中测定了酸度对幽门螺杆菌脲酶结合位点的影响。黏附的脲酶表现出高度稳定性，表明变性酶在细菌在胃黏液酸化区室中的持续存在中起关键作用。
