使用¹⁹F核磁共振光谱、高效液相色谱-核磁共振光谱和高效液相色谱-质谱法鉴定和定量大鼠尿液中2,3,5,6-四氟-4-三氟甲基苯胺的代谢产物。
Identification and quantification of metabolites of 2,3,5,6-tetrafluoro-4-trifluoromethylaniline in rat urine using 19F nuclear magnetic resonance spectroscopy, high-performance liquid chromatography-nuclear magnetic resonance spectroscopy and high-performance liquid chromatography-mass spectrometry.
作者信息
Scarfe G B, Clayton E, Wilson I D, Nicholson J K
机构信息
Biological Chemistry, Division of Biomedical Sciences, Imperial College of Science, Technology and Medicine, South Kensington, London, UK.
出版信息
J Chromatogr B Biomed Sci Appl. 2000 Oct 1;748(1):311-9. doi: 10.1016/s0378-4347(00)00321-2.
The urinary excretion profile and identity of the metabolites of 2,3,5,6-tetrafluoro-4-triflouromethylaniline, following i.p. administration to the rat at 50 mg kg(-1), were determined using a combination of 19F-NMR, HPLC-NMR and HPLC-MS. A total of 38% of the dose was eliminated in the urine as five metabolites. The major routes of metabolism were N-glucuronidation, sulfation and oxidation with a significant amount of metabolic defluorination to give a number of ortho-ring hydroxylated metabolites. The oxidised metabolites were excreted as glucuronide and/or sulfate conjugates.
以50mg/kg的剂量腹腔注射给予大鼠后，使用19F-NMR、HPLC-NMR和HPLC-MS联用技术测定了2,3,5,6-四氟-4-三氟甲基苯胺的尿排泄谱及其代谢产物的结构。给药剂量的38%以五种代谢产物的形式经尿液排出。主要代谢途径为N-葡萄糖醛酸化、硫酸化和氧化，同时伴有大量的代谢性脱氟反应，生成多种邻位环羟基化代谢产物。氧化代谢产物以葡萄糖醛酸和/或硫酸酯结合物的形式排泄。
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