Cserháti T, Forgács E, Morais M H, Mota T, Ramos A
Institute of Chemistry, Chemical Research Centre, Hungarian Academy of Sciences, Budapest.
J Chromatogr A. 2000 Oct 27;896(1-2):69-73. doi: 10.1016/s0021-9673(00)00580-x.
The performance of reversed-phase thin-layer (RP-TLC) and reversed-phase high-performance liquid chromatography (RP-HPLC) was compared for the separation and determination of the colour pigments of chili (Capsicum frutescens) powder using a wide variety of eluent systems. No separation of pigments was achieved in RP-TLC, however, it was established that tetrahydrofuran shows an unusually high solvent strength. RP-HPLC using water-methanol-acetonitrile gradient elution separated the chili pigments in many fractions. Diode array detection (DAD) indicated that yellow pigments are eluted earlier than the red ones and chili powder contains more yellow pigments than common paprika powders. It was established that the very different absorption spectra of pigments make the use of DAD necessary.
比较了反相薄层色谱（RP-TLC）和反相高效液相色谱（RP-HPLC）在使用多种洗脱系统分离和测定辣椒粉（辣椒）色素方面的性能。在RP-TLC中未实现色素的分离，然而，已确定四氢呋喃显示出异常高的溶剂强度。使用水-甲醇-乙腈梯度洗脱的RP-HPLC将辣椒色素分离为多个馏分。二极管阵列检测（DAD）表明，黄色色素比红色色素洗脱得早，并且辣椒粉中的黄色色素比普通辣椒粉中的更多。已确定色素非常不同的吸收光谱使得有必要使用DAD。
