内含子剪接增强子结合U1小核核糖核蛋白颗粒以增强剪接并选择5'剪接位点。
An intronic splicing enhancer binds U1 snRNPs to enhance splicing and select 5' splice sites.
作者信息
McCullough A J, Berget S M
机构信息
Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
出版信息
Mol Cell Biol. 2000 Dec;20(24):9225-35. doi: 10.1128/MCB.20.24.9225-9235.2000.
Abstract
Intronic G triplets are frequently located adjacent to 5' splice sites in vertebrate pre-mRNAs and have been correlated with splicing efficiency and specificity via a mechanism that activates upstream 5' splice sites in exons containing duplicated sites (26). Using an intron dependent upon G triplets for maximal activity and 5' splice site specificity, we determined that these elements bind U1 snRNPs via base pairing with U1 RNA. This interaction is novel in that it uses nucleotides 8 to 10 of U1 RNA and is independent of nucleotides 1 to 7. In vivo functionality of base pairing was documented by restoring activity and specificity to mutated G triplets through compensating U1 RNA mutations. We suggest that the G-rich region near vertebrate 5' splice sites promotes accurate splice site recognition by recruiting the U1 snRNP.
摘要
内含子G三联体在脊椎动物前体mRNA中常位于5'剪接位点附近，并通过一种机制与剪接效率和特异性相关，该机制可激活含有重复位点的外显子中的上游5'剪接位点（26）。我们使用一个依赖G三联体实现最大活性和5'剪接位点特异性的内含子，确定这些元件通过与U1 RNA碱基配对结合U1 snRNP。这种相互作用很新颖，因为它利用U1 RNA的第8至10位核苷酸，且独立于第1至7位核苷酸。通过补偿性U1 RNA突变恢复突变G三联体的活性和特异性，证明了碱基配对在体内的功能。我们认为，脊椎动物5'剪接位点附近富含G的区域通过招募U1 snRNP促进准确的剪接位点识别。
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