Dong Z, Ghabrial M, Katar M, Fridman R, Berk R S
Departments of Immunology and Microbiology and. Pathology, Wayne State University School of Medicine, Detroit, Michigan, USA.
Invest Ophthalmol Vis Sci. 2000 Dec;41(13):4189-94.
To establish the presence of membrane-type matrix metalloproteinases (MT-MMPs) in the cornea and their expression in naive and immunized mice intracorneally infected with Pseudomonas aeruginosa.
Naive (unimmunized) and immunized C57BL/6J mice were infected with P. aeruginosa, and gene expression of MT-MMPs were detected by RT-PCR. Immunoblot analysis and immunostaining were also used to characterize the MT-MMP response in both sets of animals.
Expression of MT1-MMP, MT2-MMP, and MT3-MMP (MMP 14, 15, and 16) was detected by RT-PCR and immunoblot analysis. Of the three MT-MMPs detected, MT1-MMP exhibited the greatest expression at protein levels. In general, a bell-shaped curve was obtained for each of the MT-MMPs in naive mice, but all of them showed much less expression in the immunized mice. MT1-MMP was localized in the epithelial tissue of the cornea, whereas MT2-MMP and MT3-MMP were mainly found in the interface between the epithelium and substantia propria.
MT1-MMP was detected and expressed to a greater extent in naive mice than MT2-MMP and MT3-MMP. Peak expression of all three MT-MMPs showed a good correlation with the overall inflammatory response.
确定角膜中膜型基质金属蛋白酶（MT-MMPs）的存在及其在角膜内感染铜绿假单胞菌的未免疫和免疫小鼠中的表达。
未免疫和免疫的C57BL/6J小鼠感染铜绿假单胞菌，通过逆转录聚合酶链反应（RT-PCR）检测MT-MMPs的基因表达。免疫印迹分析和免疫染色也用于表征两组动物中MT-MMP的反应。
通过RT-PCR和免疫印迹分析检测到MT1-MMP、MT2-MMP和MT3-MMP（MMP 14、15和16）的表达。在检测到的三种MT-MMP中，MT1-MMP在蛋白质水平上表现出最高的表达。总体而言，未免疫小鼠中每种MT-MMP均呈现钟形曲线，但它们在免疫小鼠中的表达均明显降低。MT1-MMP定位于角膜上皮组织，而MT2-MMP和MT3-MMP主要存在于上皮与固有层之间的界面。
与MT2-MMP和MT3-MMP相比，未免疫小鼠中检测到MT1-MMP且表达程度更高。所有三种MT-MMP的峰值表达与整体炎症反应具有良好的相关性。
