Voltchek N, Gupalova T, Totolyan A
Institute of Experimental Medicine, St. Petersburg, Russia.
Folia Microbiol (Praha). 1999;44(6):735-6. doi: 10.1007/BF02825672.
PCR generated fragments of the protein G gene from three GCS and GGS strains belonging to different G types had been cloned. The resulting PCR products were cloned into E. coli using expression vector pQE31. The clones, producing IgG-binding peptides were selected. Recombinant plasmids carried different inserts and encoded proteins of different size and with different binding properties.
已克隆出属于不同G型的三株GCS和GGS菌株中蛋白G基因的PCR扩增片段。使用表达载体pQE31将所得PCR产物克隆到大肠杆菌中。筛选出产生IgG结合肽的克隆。重组质粒携带不同的插入片段，编码不同大小且具有不同结合特性的蛋白质。
