Foderà R, Caneva R, Canzonetta C, Savino M
CNR Centro di Studio per gli Acidi Nucleici, Università di Roma La Sapienza, Rome, Italy.
Nucleosides Nucleotides Nucleic Acids. 2000 Aug;19(8):1231-40. doi: 10.1080/15257770008033046.
Using the circular dichroism spectra induced in the visible by the binding to the minor groove of DNA, we found that Hoechst 33258 is able to occupy its specific sites even when these are located inside the nucleosome structure. This high accessibility of the DNA in the nucleosome is not modified by the removal of the amino-terminal domains of the octamer histones and is not reduced by the presence of linker histone. Interesting and reasonable differences were found in the association constants.
利用与DNA小沟结合在可见光区诱导产生的圆二色光谱，我们发现，即使Hoechst 33258的特异性位点位于核小体结构内部，它也能够占据这些位点。核小体中DNA的这种高可及性不会因八聚体组蛋白氨基末端结构域的去除而改变，也不会因连接组蛋白的存在而降低。在结合常数方面发现了有趣且合理的差异。
