Giordano C, Pedone F, Fattibene P, Cellai L
Centro di Chimica del Farmaco, CNR, Dipartimento di Studi Farmaceutici, Università La Sapienza, Rome, Italy.
Nucleosides Nucleotides Nucleic Acids. 2000 Aug;19(8):1301-10. doi: 10.1080/15257770008033053.
In order to achieve an EPR sensitive probe for DNA, 3-carboxy-Proxyl free radical was linked to O-6 of dG through a five-atoms-tether. The modified base was incorporated into a 30-mer ODN, then annealed to its complementary DNA strand. Hydrodynamic parameters show only a slight destabilization with respect to the equivalent unlabeled hybrid. EPR could monitor the hybrid formation showing a progressive enlargement of the upfield signal in passing from the labeled ss- to the ds-30-mer.
为了获得一种对DNA具有电子顺磁共振（EPR）敏感性的探针，将3-羧基-脯氨酰自由基通过一个五原子连接链连接到dG的O-6位上。将修饰后的碱基掺入到一条30个碱基的寡脱氧核苷酸（ODN）中，然后与它的互补DNA链退火。流体动力学参数表明，相对于等效的未标记杂交体，其稳定性仅有轻微下降。EPR能够监测杂交体的形成，从标记的单链30聚体到双链30聚体的过程中，EPR信号的上场信号逐渐增大。
