利用重组痘苗病毒将犬新孢子虫表面蛋白NcSRS2（Nc-p43）递送至小鼠体内。

Delivery of Neospora caninum surface protein, NcSRS2 (Nc-p43), to mouse using recombinant vaccinia virus.

作者信息

Nishikawa Y, Kousaka Y, Fukumoto S, Xuan X, Nagasawa H, Igarashi I, Fujisaki K, Otsuka H, Mikami T

机构信息

The National Research Center for Protozoan Diseases, Obihiro University, Hokkaido, Japan.

出版信息

Parasitol Res. 2000 Nov;86(11):934-9. doi: 10.1007/s004360000267.

DOI:10.1007/s004360000267

PMID:11097303

Abstract

In order to develop a vaccine against Neospora caninum in dogs and cattle, we constructed a recombinant vaccinia virus expressing the N. caninum surface protein, NcSRS2 (Nc-p43). Monoclonal antibodies to NcSRS2 and anti-N. caninum tachyzoite mouse serum recognized the NcSRS2 expressed by the recombinant vaccinia virus. In addition, recombinant NcSRS2 was transported to the cell surface. Mice infected with the recombinant virus predominantly produced IgG1 antibody (Ab) to N. caninum, rather than producing IgG2a Ab. Moreover, splenocytes from mice infected with the recombinant virus proliferated in the presence of the N. caninum antigen. Mice immunized with the recombinant virus gave rise to humoral and cellular immune responses to N. caninum tachyzoites. This study showed that a recombinant vaccinia virus expressing NcSRS2 might be useful for the production of a live vaccine against N. caninum infection.

摘要

为了研发针对犬和牛新孢子虫的疫苗，我们构建了一种表达新孢子虫表面蛋白NcSRS2（Nc-p43）的重组痘苗病毒。抗NcSRS2单克隆抗体和抗新孢子虫速殖子小鼠血清可识别重组痘苗病毒表达的NcSRS2。此外，重组NcSRS2被转运至细胞表面。感染重组病毒的小鼠主要产生针对新孢子虫的IgG1抗体，而非产生IgG2a抗体。而且，感染重组病毒小鼠的脾细胞在新孢子虫抗原存在的情况下发生增殖。用重组病毒免疫的小鼠对新孢子虫速殖子产生了体液免疫和细胞免疫反应。本研究表明，表达NcSRS2的重组痘苗病毒可能有助于生产针对新孢子虫感染的活疫苗。