Zeibecoglou K, Ying S, Meng Q, Kay A B, Robinson D S, Papageorgiou N
Allergy and Clinical Immunology, Imperial College School of Medicine, National Heart and Lung Institute, London, UK.
Allergy. 2000 Nov;55(11):1042-8. doi: 10.1034/j.1398-9995.2000.00764.x.
The chemokine eotaxin has been implicated in airway eosinophilia in atopic asthma. We have compared airway eosinophils and eotaxin expression in induced sputum from well-matched atopic and nonatopic asthmatics.
Eosinophil numbers, eosinophil cationic protein (ECP), and the expression of eotaxin were examined in induced sputum from atopic asthmatics (AA = 11), nonatopic asthmatics (NAA = 11), and atopic (AC = 12) and normal (NC = 10) controls. Slides were prepared for differential cell counts by Romanowsky stain, and ECP levels were measured by RIA. Eotaxin expression was detected by in situ hybridization, with 35S-labelled riboprobes and immunocytochemistry.
The numbers of eosinophils and ECP concentration were increased in the sputum of AA and NAA compared with AC and NC (P < 0.05). The numbers of eotaxin mRNA+ and immunoreactive cells were increased in NAA, but not AA, when compared with controls (P < 0.05). Eotaxin immunoreactive cells in NAA were significantly higher than in AA (P < 0.05). Eotaxin was expressed predominantly by macrophages, eosinophils, and epithelial cells. In NAA, but not AA, the numbers of eotaxin mRNA+ cells were correlated with histamine PC20 (r = -0.81, P < 0.01) and eosinophil numbers in sputum (r = 0.7, P < 0.05).
Eotaxin production by macrophages, eosinophils, and epithelial cells may play a more pronounced role in airway eosinophilia in nonatopic than in atopic asthma.
趋化因子嗜酸性粒细胞趋化因子与特应性哮喘患者气道嗜酸性粒细胞增多有关。我们比较了病情匹配的特应性哮喘患者和非特应性哮喘患者诱导痰中的气道嗜酸性粒细胞及嗜酸性粒细胞趋化因子表达情况。
检测了特应性哮喘患者(AA = 11例)、非特应性哮喘患者(NAA = 11例)、特应性对照者(AC = 12例)和正常对照者(NC = 10例)诱导痰中的嗜酸性粒细胞数量、嗜酸性粒细胞阳离子蛋白(ECP)以及嗜酸性粒细胞趋化因子的表达。通过罗曼诺夫斯基染色制备玻片进行细胞分类计数,采用放射免疫分析法测定ECP水平。使用35S标记的核糖探针通过原位杂交及免疫细胞化学检测嗜酸性粒细胞趋化因子表达。
与AC组和NC组相比,AA组和NAA组痰液中的嗜酸性粒细胞数量及ECP浓度升高(P < 0.05)。与对照组相比,NAA组而非AA组中嗜酸性粒细胞趋化因子mRNA+细胞及免疫反应性细胞数量增加(P < 0.叭)。NAA组中嗜酸性粒细胞趋化因子免疫反应性细胞显著高于AA组(P < 0.05)。嗜酸性粒细胞趋化因子主要由巨噬细胞、嗜酸性粒细胞和上皮细胞表达。在NAA组而非AA组中,嗜酸性粒细胞趋化因子mRNA+细胞数量与组胺PC20(r = -0.81,P < 0.01)及痰液中嗜酸性粒细胞数量(r = 0.7,P < 0.05)相关。
巨噬细胞、嗜酸性粒细胞和上皮细胞产生的嗜酸性粒细胞趋化因子在非特应性哮喘患者气道嗜酸性粒细胞增多中可能比在特应性哮喘中发挥更显著的作用。