Minuk G Y, Orr P S, Brown R, Macdonald S, Chaudhary R K, Temple P
Liver Diseases Unit, University of Manitoba, Winnipeg, Canada.
J Hepatol. 2000 Nov;33(5):781-4. doi: 10.1016/s0168-8278(00)80310-4.
BACKGROUND/AIMS: Previous cross-sectional data suggested that chronic hepatitis B viral (HBV) infections in the Canadian Inuit were inactive. The aim of this study was to confirm these findings and document the prevalence of the subsequently described "pre-core mutant" variant of HBV in this population.
We obtained sera from residents of five remote Canadian Inuit communities. Residents were selected if they were known to be hepatitis B surface antigen (HBsAg) positive or had a history of liver disease. HBV serology, HBV-DNA, and pre-core mutant testing were performed by commercially available assays, polymerase chain reaction (PCR) and direct sequencing of the viral genome, respectively.
Sera were obtained from 176/266 (66%) of selected individuals. Thirty-eight (22%) were HBsAg positive and 16 (9.1%) anti-HBs positive. Of HBsAg positive carriers 25/38 (66%) were male as compared to 68/138 (49%) of the remaining individuals (p<0.05). Of 37 HBsAg positive carriers, none were HBeAg positive, 36 (97%) anti-HBe positive and one (3%) HBeAg and anti-HBe negative. Liver enzyme and function tests were normal in all cases. 30/37 (81%) HBsAg positive carriers were HBV-DNA positive and 26/30 (87%) were pre-core mutant positive.
The majority of HBV infections in community-based Canadian Inuit are inactive and the prevalence of pre-core mutant infections is the highest reported to date.
背景/目的：既往横断面数据提示，加拿大因纽特人中的慢性乙型肝炎病毒（HBV）感染呈非活动状态。本研究旨在证实这些发现，并记录该人群中随后描述的HBV“前核心突变体”变体的流行情况。
我们从加拿大五个偏远因纽特人社区的居民中获取血清。若居民已知为乙型肝炎表面抗原（HBsAg）阳性或有肝病病史，则被纳入研究。分别通过商用检测方法、聚合酶链反应（PCR）以及病毒基因组直接测序进行HBV血清学、HBV-DNA和前核心突变体检测。
从选定个体中的176/266（66%）获取了血清。38人（22%）HBsAg阳性，16人（9.1%）抗-HBs阳性。在HBsAg阳性携带者中，25/38（66%）为男性，而其余个体中这一比例为68/138（49%）（p<0.05）。在37名HBsAg阳性携带者中，无HBeAg阳性者，36人（97%）抗-HBe阳性，1人（3%）HBeAg和抗-HBe均阴性。所有病例的肝酶和肝功能检查均正常。30/37（81%）HBsAg阳性携带者HBV-DNA阳性，26/30（87%）前核心突变体阳性。
以社区为基础的加拿大因纽特人中，大多数HBV感染呈非活动状态，且前核心突变体感染的流行率是迄今报告的最高水平。
