Papazisis K T, Zambouli D, Kimoundri O T, Papadakis E S, Vala V, Geromichalos G D, Voyatzi S, Markala D, Destouni E, Boutis L, Kortsaris A H
Theagenion Cancer Hospital, 54007, Thessaloniki, Greece.
Cancer Lett. 2000 Nov 10;160(1):107-13. doi: 10.1016/s0304-3835(00)00569-3.
Genistein, is a natural isoflavone compound with a potent activity against protein tyrosine kinases. The leukemic cell line, K562, is a bcr/abl (Philadelphia chromosome) positive cell line that is resistant to DNA-damaging agents, including gamma-irradiation. Treatment with genistein increased apoptosis and promoted G2-phase arrest in the non-apoptotic population of the gamma-irradiated K562 cells. Irradiated cells that survived 72 h after the irradiation had a normal distribution in cell cycle, whilst genistein treatment kept cells arrested in the G2-phase, decreased the S-phase fraction and suppressed DNA-synthesis. Taken together, our results show that genistein augments apoptotic cell death after gamma-irradiation in K562 cells and this result cannot be attributed to abrogation of the G2/M checkpoint.
染料木黄酮是一种天然异黄酮化合物，对蛋白酪氨酸激酶具有强大的活性。白血病细胞系K562是一种bcr/abl（费城染色体）阳性细胞系，对包括γ射线在内的DNA损伤剂具有抗性。用染料木黄酮处理可增加γ射线照射的K562细胞非凋亡群体中的细胞凋亡并促进G2期阻滞。照射后存活72小时的受照细胞在细胞周期中分布正常，而染料木黄酮处理使细胞停滞在G2期，降低S期比例并抑制DNA合成。综上所述，我们的结果表明，染料木黄酮可增强γ射线照射后K562细胞中的凋亡细胞死亡，且这一结果不能归因于G2/M检查点的消除。
