Dulieu C, Moll M, Boudrant J, Poncelet D
LFBI, ENSAIA, 2 avenue de la Forêt de Haye, BP 172, F-54505 Vandoeuvre les Nancy Cedex, France.
Biotechnol Prog. 2000 Nov-Dec;16(6):958-65. doi: 10.1021/bp000128k.
The use of the enzyme alpha-acetolactate decarboxylase allows the acceleration of beer fermentation/maturation because it shunts diacetyl formation, whose elimination is the rate-limiting step of the process. To obtain a cost reduction by using this exogenous enzyme, we propose a new process involving recoverable encapsulated alpha-acetolactate decarboxylase. The performance of traditional and new processes was investigated by a modeling approach. A simple model, focused on alpha-acetolactate and diacetyl profiles during beer fermentation, was set up. The simulated profiles are consistent with literature data. This study shows also that encapsulated alpha-acetolactate decarboxylase allows the acceleration of beer fermentation as efficiently as free alpha-acetolactate decarboxylase. The advantage of immobilized alpha-acetolactate decarboxylase versus free enzyme is that it is recoverable and reusable, which means a process cost reduction.
使用α-乙酰乳酸脱羧酶可加速啤酒发酵/成熟过程，因为它能分流双乙酰的形成，而双乙酰的去除是该过程的限速步骤。为了通过使用这种外源酶来降低成本，我们提出了一种涉及可回收包封的α-乙酰乳酸脱羧酶的新工艺。通过建模方法研究了传统工艺和新工艺的性能。建立了一个简单的模型，重点关注啤酒发酵过程中α-乙酰乳酸和双乙酰的变化情况。模拟结果与文献数据一致。本研究还表明，包封的α-乙酰乳酸脱羧酶与游离的α-乙酰乳酸脱羧酶一样能有效地加速啤酒发酵。固定化α-乙酰乳酸脱羧酶相对于游离酶的优势在于它可回收和重复使用，这意味着工艺成本降低。
