Kanai M, Kurobe M, Yamazaki Y
Department of Surgery, Jikei University School of Medicine, Tokyo, Japan.
J Pediatr Surg. 2000 Dec;35(12):1701-5. doi: 10.1053/jpsu.2000.19216.
The aim of this study was to establish a model of bacterial translocation (BT) in neuroblastoma-bearing mice.
A suspension of 1 x 10(6) cells of the murine neuroblastoma cell line C1300 was injected subcutaneously into the thighs of 8-week-old female A/J mice, which were then killed after 7, 14, and 21 days. Some of the mice were given 1-microm or 2-microm fluorescein-labeled latex beads in their drinking water for 7 days before being killed. Mesenteric lymph nodes (MLNs) were aseptically removed and cultured for 72 hours at 37 degrees C. Segments of distal ileum were obtained for histologic examination. Samples of venous blood were obtained for laboratory tests.
Tumors were found at the injection sites on days 14 and 21 after C1300 injection. Although tumors were not found in 7 days, significantly high number of 1-microm latex beads were detected in MLNs compared with the control, and the number increased with tumor growth. The number of 2-microm latex beads was significantly higher on days 14 and 21. The percentage of mice with MLN cultures positive were significantly higher on day 14, and the percentage increased along with tumor growth. On day 21 after C1300 injection, body weight loss and anemia were observed, and histologic findings of the terminal ileum showed mucosal edema and villous thinning. Serum levels of interleukin (IL)-6 were significantly higher in mice killed 14 and 21 days after injection.
The results suggest that BT from the gut to MLNs may occur in neuroblastoma C1300-bearing mice, and it increases along with tumor growth. Even in the early stage of malignancy, particles as small as 1 microm may translocate from the gut to MLNs.
本研究旨在建立荷神经母细胞瘤小鼠的细菌移位（BT）模型。
将1×10⁶个小鼠神经母细胞瘤细胞系C1300的细胞悬液皮下注射到8周龄雌性A/J小鼠的大腿中，然后在7天、14天和21天后处死小鼠。部分小鼠在处死前7天在饮用水中给予1微米或2微米荧光素标记的乳胶珠。无菌切除肠系膜淋巴结（MLN）并在37℃培养72小时。获取回肠末端段进行组织学检查。采集静脉血样本进行实验室检测。
在注射C1300后第14天和第21天，在注射部位发现肿瘤。虽然在7天时未发现肿瘤，但与对照组相比，在MLN中检测到的1微米乳胶珠数量显著增多，且该数量随肿瘤生长而增加。在第14天和第21天，2微米乳胶珠的数量显著更高。MLN培养阳性的小鼠百分比在第14天显著更高，且该百分比随肿瘤生长而增加。在注射C1300后第21天，观察到体重减轻和贫血，回肠末端的组织学检查结果显示黏膜水肿和绒毛变薄。注射后14天和21天处死的小鼠血清白细胞介素（IL）-6水平显著更高。
结果表明，荷神经母细胞瘤C1300小鼠可能发生从肠道到MLN的BT，且其随肿瘤生长而增加。即使在恶性肿瘤的早期阶段，小至1微米的颗粒也可能从肠道转移至MLN。
