Berg A I, Noks P P, Kononenko A A, Frolov E N, Khrymova I N
Mol Biol (Mosk). 1979 Jan-Feb;13(1):81-9.
In reaction centres of photosynthetic membranes of R, rubrum the efficiency of redox interactions of the photoreduced primary electron acceptor with secondary acceptors and photooxidized bacteriochlorophyll has a marked and reversible dependence on temperature over the range from -20 degrees to -80 degrees. Similar temperature dependences were observed for correlation times of the rotational diffusion of a spin probe bound to the hydrophobic region of the membrane and of a spin label bound to SH-groups of the protein constituent of the membrane, as well as for characteristics of resonance absorption of the Mössbauer probe (57Fe), embedded in the membrane by biosynthesis. Using the same indicators of intramolecular mobility, a correlation has been observed between photoactivity and structure of the membrane in experiments involving hydratation changes of samples. The data suggest the existence of a close interrelationship between the conformational mobility of the constituents of the photosynthetic membrane and their specific reaction capacity.
在红假单胞菌光合膜的反应中心，光还原的初级电子受体与次级受体以及光氧化的细菌叶绿素之间的氧化还原相互作用效率，在-20摄氏度至-80摄氏度的温度范围内对温度有显著且可逆的依赖性。对于结合到膜疏水区域的自旋探针以及结合到膜蛋白质成分SH基团的自旋标记的旋转扩散相关时间，以及通过生物合成嵌入膜中的穆斯堡尔探针（57Fe）的共振吸收特性，也观察到了类似的温度依赖性。在涉及样品水合变化的实验中，使用相同的分子内流动性指标，观察到了光活性与膜结构之间的相关性。数据表明光合膜成分的构象流动性与其特定反应能力之间存在密切的相互关系。
