Ragan M A, MacCallum G S, Murphy C A, Cannone J J, Gutell R R, McGladdery S E
Institute for Marine Biosciences, National Research Council of Canada, Halifax, Nova Scotia.
Dis Aquat Organ. 2000 Sep 28;42(3):185-90. doi: 10.3354/dao042185.
Biomass of the protistan parasite QPX (quahaug parasite X) of hard-shell clam Mercenaria mercenaria was enriched from in vitro culture. The nuclear gene encoding the 18S RNA of the small-subunit ribosomal (ssu-rDNA) was recovered using the polymerase chain reaction (PCR) and sequenced. Phylogenetic analysis clearly showed that QPX is a member of phylum Labyrinthulomycota, within which it appears as a specific relative of Thraustochytrium pachydermum. These results confirm the provisional assignment of QPX to the Labyrinthulomycota made previously on the basis of morphological and ultrastructural characters found in some, but not all, geographic isolates.
硬壳蛤(Mercenaria mercenaria)的原生动物寄生虫QPX(硬壳蛤寄生虫X)的生物量是从体外培养物中富集得到的。利用聚合酶链反应(PCR)回收了编码小亚基核糖体18S RNA的核基因(ssu-rDNA)并进行了测序。系统发育分析清楚地表明,QPX是网粘菌门的一个成员,在该门中它似乎是厚皮破囊壶菌(Thraustochytrium pachydermum)的一个特定近缘种。这些结果证实了先前根据在部分(而非全部)地理分离株中发现的形态学和超微结构特征而将QPX临时归入网粘菌门的分类。