Lai C L, Chao Y C, Chen Y C, Liao C S, Chen M C, Liu Y C, Yin S J
Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China.
Alcohol Clin Exp Res. 2000 Nov;24(11):1625-32.
Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are the principal enzymes responsible for ethanol metabolism in humans. The stomach is involved in the metabolism of alcohol during absorption. Conflicting reports exist with regard to the influence of sex and age on the activity of ADH in the human gastric mucosa. The purpose of the present study was to determine the effects of age and sex on the expression pattern and activities of stomach ADH and ALDH.
A total of 115 endoscopic gastric biopsy specimens were investigated from Han Chinese men (n = 70) and women (n = 45) aged 20-79 years with approximately even distribution among 10-year age intervals. The expression patterns of ADH and ALDH were identified by isoelectric focusing, and the activities were assayed spectrophotometrically.
The expression patterns of gastric ADH and ALDH remained unchanged with respect to sex and age. At 33 mM or 500 mM ethanol, pH 7.5, the ADH activities did not differ significantly among the various age groups or between men and women. At 200 microM or 20 mM acetaldehyde, the ALDH activities did not differ significantly in relation to sex and age. No correlations were found between the ADH or ALDH activities at both the high and low substrate concentrations and the ages in men and women.
The results indicate that there is no significant effect of either sex or age on the expression pattern and activity of ADH and ALDH in the human gastric mucosa. The stomach ADH seems unlikely to account for possible variations in the first-pass metabolism of alcohol with regard to sex and age.
乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）是人体内负责乙醇代谢的主要酶。胃在酒精吸收过程中参与其代谢。关于性别和年龄对人胃黏膜中ADH活性的影响，存在相互矛盾的报道。本研究的目的是确定年龄和性别对胃ADH和ALDH的表达模式及活性的影响。
共研究了115例来自20 - 79岁汉族男性（n = 70）和女性（n = 45）的内镜胃活检标本，在10年年龄间隔内分布大致均匀。通过等电聚焦鉴定ADH和ALDH的表达模式，并用分光光度法测定活性。
胃ADH和ALDH的表达模式在性别和年龄方面保持不变。在33 mM或500 mM乙醇、pH 7.5条件下，不同年龄组之间或男女之间的ADH活性无显著差异。在200 μM或20 mM乙醛条件下，ALDH活性在性别和年龄方面也无显著差异。在高底物浓度和低底物浓度下，ADH或ALDH活性与男性和女性的年龄之间均未发现相关性。
结果表明，性别和年龄对人胃黏膜中ADH和ALDH的表达模式及活性均无显著影响。胃ADH似乎不太可能解释酒精首过代谢在性别和年龄方面可能存在的差异。
