Hirata T, Merrill-Skoloff G, Aab M, Yang J, Furie B C, Furie B
Center for Hemostasis and Thrombosis Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
J Exp Med. 2000 Dec 4;192(11):1669-76. doi: 10.1084/jem.192.11.1669.
P-selectin glycoprotein ligand 1 (PSGL-1) is a sialomucin expressed on leukocytes that mediates neutrophil rolling on the vascular endothelium. Here, the role of PSGL-1 in mediating lymphocyte migration was studied using mice lacking PSGL-1. In a contact hypersensitivity model, the infiltration of CD4(+) T lymphocytes into the inflamed skin was reduced in PSGL-1-deficient mice. In vitro-generated T helper (Th)1 cells from PSGL-1-deficient mice did not bind to P-selectin and migrated less efficiently into the inflamed skin than wild-type Th1 cells. To assess the role of PSGL-1 in P- or E-selectin-mediated migration of Th1 cells, the cells were injected into E- or P-selectin-deficient mice. PSGL-1-deficient Th1 cells did not migrate into the inflamed skin of E-selectin-deficient mice, indicating that PSGL-1 on Th1 cells is the sole ligand for P-selectin in vivo. In contrast, PSGL-1-deficient Th1 cells migrated into the inflamed skin of P-selectin-deficient mice, although less efficiently than wild-type Th1 cells. This E-selectin-mediated migration of PSGL-1-deficient or wild-type Th1 cells was not altered by injecting a blocking antibody to L-selectin. These data provide evidence that PSGL-1 on Th1 cells functions as one of the E-selectin ligands in vivo.
P选择素糖蛋白配体1（PSGL-1）是一种在白细胞上表达的唾液粘蛋白，可介导中性粒细胞在血管内皮上滚动。在此，利用缺乏PSGL-1的小鼠研究了PSGL-1在介导淋巴细胞迁移中的作用。在接触性超敏反应模型中，PSGL-1缺陷小鼠中CD4(+) T淋巴细胞向炎症皮肤的浸润减少。与野生型Th1细胞相比，来自PSGL-1缺陷小鼠的体外生成的辅助性T（Th）1细胞不与P选择素结合，且向炎症皮肤的迁移效率较低。为了评估PSGL-1在P或E选择素介导的Th1细胞迁移中的作用，将这些细胞注射到E或P选择素缺陷小鼠中。PSGL-1缺陷的Th1细胞不迁移到E选择素缺陷小鼠的炎症皮肤中，这表明Th1细胞上的PSGL-1是体内P选择素的唯一配体。相反，PSGL-1缺陷的Th1细胞迁移到P选择素缺陷小鼠的炎症皮肤中，尽管效率低于野生型Th1细胞。注射L选择素阻断抗体不会改变PSGL-1缺陷或野生型Th1细胞的这种E选择素介导的迁移。这些数据提供了证据，表明Th1细胞上的PSGL-1在体内作为E选择素配体之一发挥作用。
