Pupo Antúnez M, Alvarez Vera M, Soto Hernández M, Rodríguez Sánchez H, Rodríguez Díaz R, Morier Díaz L, Guzmán Tirado M G
Instituto de Medicina Tropical Pedro Kourí, Marianao, Ciudad de La Habana, Cuba.
Rev Cubana Med Trop. 2000 May-Aug;52(2):119-25.
These types of monoclonal antibodies 8E8, 3F7 and 1E9 to dengue 4 virus H-241 strain. These monoclonal antibodies show various patterns of reactivity to the four dengue serotypes and different antigen preparations of serotype 4 when they were tested in various serological methods. The monoclonal antibody 8E8 exhibited a specificity of serotype (D-2; by hemagglutination inhibition); subcomplex (D-2 and D-4 by immunofluorescence) and complex (by immunoperoxidase technique). It was able to neutralize by 80% homologous virus and it turned out to be the only reactive monoclonal antibody in the complement fixation test. The monoclonal 3F7 did not react to by hemagglutination inhibition, recognized serotypes D-1, D-2, D-3 and D-4 by immunofluorescence and only serotypes D1 and D4 by immunoperoxidase technique but it was unable to neutralize the homologous virus. The 1E9 antibody was reactive to serotypes D1, D-2, D-3 and D-4 only by hemagglutination inhibition and neutralized serotype D-4. All the monoclonal antibodies were able to react to various dengue antigens through an ELISA of double antibody and showed fluorescent activity against 38th pass in Beagle dog kidney culture; however, they could not react to a D-4 recombinant antigen.
这些针对登革4型病毒H - 241株的单克隆抗体8E8、3F7和1E9。当采用各种血清学方法进行检测时，这些单克隆抗体对四种登革血清型以及血清型4的不同抗原制剂表现出不同的反应模式。单克隆抗体8E8表现出血清型特异性（通过血凝抑制试验为D - 2型）；亚复合物特异性（通过免疫荧光法为D - 2和D - 4型）以及复合物特异性（通过免疫过氧化物酶技术）。它能够中和80%的同源病毒，并且在补体结合试验中是唯一有反应的单克隆抗体。单克隆抗体3F7通过血凝抑制试验无反应，通过免疫荧光法可识别血清型D - 1、D - 2、D - 3和D - 4，通过免疫过氧化物酶技术仅识别血清型D1和D4，但它不能中和同源病毒。1E9抗体仅通过血凝抑制试验对血清型D1、D - 2、D - 3和D - 4有反应，并能中和血清型D - 4。所有单克隆抗体都能够通过双抗体酶联免疫吸附测定法与各种登革抗原发生反应，并且对比格犬肾细胞培养第38代病毒表现出荧光活性；然而，它们不能与D - 4重组抗原发生反应。
