Dutschmann M, Wilson R J, Paton J F
Department of Physiology, School of Medical Sciences, University of Bristol, UK.
Auton Neurosci. 2000 Oct 30;84(1-2):19-29. doi: 10.1016/S1566-0702(00)00177-6.
In neonatal animals in vitro preparations have been employed widely to study the central control of respiration. These preparations have limitations in that reflex afferent inputs and kinesiological studies cannot be performed. Here, we describe an alternative in situ experimental model for studying both peripheral and central control of the respiratory system in neonatal rats. Using technology based on adult mammals, we introduce an intra-arterially perfused working heart-brainstem preparation (WHBP) that permits studies on eupnoeic respiration in neonatal rats from within a few hours of birth. Using this preparation we demonstrate a three-phase respiratory rhythm as revealed by the activity in phrenic and recurrent laryngeal motor nerves, the respiratory modulation of laryngeal resistance and the firing patterns of respiratory neurones recorded from the ventrolateral medulla. We conclude that the neonatal rat WHBP is an in situ preparation because it produces a respiratory rhythm similar to that of adult in vivo mammal preparations but distinct from in vitro preparations.
在新生动物中，体外制备方法已被广泛用于研究呼吸的中枢控制。这些制备方法存在局限性，即无法进行反射传入输入和运动学研究。在此，我们描述了一种用于研究新生大鼠呼吸系统外周和中枢控制的原位实验模型。利用基于成年哺乳动物的技术，我们引入了一种动脉内灌注的工作心脏-脑干制备方法（WHBP），该方法允许在新生大鼠出生后数小时内对其正常呼吸进行研究。使用该制备方法，我们通过膈神经和喉返运动神经的活动、喉阻力的呼吸调节以及从延髓腹外侧记录的呼吸神经元的放电模式，证明了一种三相呼吸节律。我们得出结论，新生大鼠WHBP是一种原位制备方法，因为它产生的呼吸节律与成年体内哺乳动物制备方法相似，但与体外制备方法不同。
