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通过薄层色谱法分离指纹残留物中可见激发的荧光成分。

Separation of visibly-excited fluorescent components in fingerprint residue by thin-layer chromatography.

作者信息

Jones N E, Davies L M, Brennan J S, Bramble S K

机构信息

Forensic Sciences Service, Metropolitan Laboratory, London, UK.

出版信息

J Forensic Sci. 2000 Nov;45(6):1286-93.

PMID:11110184
Abstract

The use of lasers for the detection of fingermarks is widespread in the forensic field. Despite this, and the fact that many studies have been conducted into the composition of fingermark residue, the components responsible for the inherent visible fluorescence remain unidentified. Traditionally compositional studies have been performed on sweat, sebum, or skin surface washes, none of which are truly representative of the situation when a fingerprint is deposited on a surface. In this paper thin-layer chromatography (TLC) has been performed on sebum-rich fingermarks laid directly onto TLC plates and an argon ion laser used to visualize the separated components. It has been found to be a robust and reproducible method for studying the fluorescent components in fingermark residue and is considered to be more realistic than other methods of sample preparation as it eliminates the chances of extraneous matter being extracted from the skin surface. Investigations into the nature of the separated compounds have also been made and the results are reported.

摘要

激光在指纹检测中的应用在法医学领域十分广泛。尽管如此,而且已有许多关于指纹残留成分的研究,但导致固有可见荧光的成分仍未明确。传统的成分研究是针对汗液、皮脂或皮肤表面清洗液进行的,这些都不能真正代表指纹沉积在表面时的实际情况。在本文中,对直接放置在薄层色谱(TLC)板上的富含皮脂的指纹进行了薄层色谱分析,并使用氩离子激光来观察分离出的成分。已发现这是一种用于研究指纹残留中荧光成分的可靠且可重复的方法,并且由于它消除了从皮肤表面提取外来物质的可能性,所以被认为比其他样品制备方法更符合实际情况。还对分离出的化合物的性质进行了研究,并报告了结果。

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