Yan Y B, Wu Q, Zhang R Q
Department of Biological Sciences and Biotechnology, Tsinghua University, 100084, Beijing, PR China.
FEMS Microbiol Lett. 2000 Dec 15;193(2):269-73. doi: 10.1111/j.1574-6968.2000.tb09435.x.
The synthesis and degradation of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-hydroxyvalerate) (P(HB-co-HV)) by Azotobacter vinelandii UWD were investigated using natural abundance solution (13)C nuclear magnetic resonance (NMR) in vivo in shake flask culture and in fermenter culture. The synthesis and the degradation of poly(3-hydroxyalkanoate)s (PHA) monomers hydroxybutyrate (HB) and hydroxyvalerate (HV) had different rates. The amount of HB and HV increased dramatically in the initial degradation stage. The results suggest that the intracellular PHA of strain UWD was the subject of dynamic metabolic processing. (13)C NMR in vivo analysis provided a rapid, easy, accurate, non-destructive method to obtain valuable information on the metabolism of PHA.
利用天然丰度溶液（13）C核磁共振（NMR）技术，在摇瓶培养和发酵罐培养的体内条件下，研究了棕色固氮菌UWD对聚（3-羟基丁酸酯）（PHB）和聚（3-羟基丁酸酯-共-羟基戊酸酯）（P（HB-co-HV））的合成与降解。聚（3-羟基链烷酸酯）（PHA）单体3-羟基丁酸（HB）和3-羟基戊酸（HV）的合成与降解速率不同。在初始降解阶段，HB和HV的量急剧增加。结果表明，菌株UWD的细胞内PHA是动态代谢过程的主体。体内（13）C NMR分析提供了一种快速、简便、准确、无损的方法，以获取有关PHA代谢的有价值信息。
