致病岛中的毒素基因：对微生物进化的影响

Toxin genes on pathogenicity islands: impact for microbial evolution.

作者信息

Dobrindt U, Janke B, Piechaczek K, Nagy G, Ziebuhr W, Fischer G, Schierhorn A, Hecker M, Blum-Oehler G, Hacker J

机构信息

Institut für Molekulare Infektionsbiologie, Würzburg, Germany.

出版信息

Int J Med Microbiol. 2000 Oct;290(4-5):307-11. doi: 10.1016/S1438-4221(00)80028-4.

DOI:10.1016/S1438-4221(00)80028-4

PMID:11111903

Abstract

Toxin-specific genes are often located on mobile genetic elements such as phages, plasmids and pathogenicity islands (PAIs). The uropathogenic E. coli strain 536 carries two alpha-hemolysin gene clusters, which are part of the pathogenicity islands I536 and II536, respectively. Using different genetic techniques, two additional PAIs were identified in the genome of the E. coli strain 536, and it is likely that further PAIs are located on the genome of this strain. Pathogenicity islands are often associated with tRNA genes. In the case of the E. coli strain 536, the PAI-associated tRNA gene leuX, which encodes a minor leucyl-tRNA, affects the expression of various virulence traits including alpha-hemolysin production. The exact mode of action of the tRNA5Leu-dependent gene expression has to be identified in the future.

摘要

毒素特异性基因通常位于移动遗传元件上，如噬菌体、质粒和致病岛（PAIs）。尿路致病性大肠杆菌菌株536携带两个α-溶血素基因簇，它们分别是致病岛I536和II536的一部分。通过不同的遗传技术，在大肠杆菌菌株536的基因组中又鉴定出了两个致病岛，并且该菌株的基因组上可能还存在更多致病岛。致病岛通常与tRNA基因相关。就大肠杆菌菌株536而言，与致病岛相关的tRNA基因leuX编码一种次要的亮氨酰-tRNA，它会影响包括α-溶血素产生在内的各种毒力性状的表达。tRNA5Leu依赖性基因表达的确切作用模式有待未来确定。