Miki H, Mio T, Nagai S, Hoshino Y, Nagao T, Kitaichi M, Izumi T
Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Japan.
Am J Respir Crit Care Med. 2000 Dec;162(6):2259-64. doi: 10.1164/ajrccm.162.6.9812029.
The aim of this study was to compare the function of lung fibroblasts obtained from surgically biopsied specimens of patients with idiopathic pulmonary fibrosis/usual interstitial pneumonia (UIP; n = 5), nonspecific interstitial pneumonia (NSIP; n = 5), and normal parts of surgically resected lungs (control; n = 5). The results showed that (1) fibroblasts obtained from UIP showed increased contractility compared with those obtained from NSIP or controls (UIP, 72.7 +/- 6.21%; NSIP, 32.8 +/- 5.46; controls, 28.5 +/- 3.51, p < 0.01 in UIP versus NSIP or control); (2) this increase in contractility was consistent with enhanced F-actin content in fibroblasts; (3) conditioned media from UIP fibroblast cultures enhanced control fibroblast contractility, whereas those obtained from NSIP or controls did not; (4) the 180 and 25 kD products representing the contractility in conditioned media were identified as fibronectin (ED-A domain) and TGF-beta1 by immunoblots, respectively; (5) the UIP-conditioned media contained higher amounts of fibronectin or TGF-beta 1 (fibronectin: UIP 289 +/- 47.1 ng/ml, NSIP 121 +/- 23.0, control 118 +/- 16.0; TGF-beta1: UIP 798 +/- 119 pg/ml, NSIP 246 +/- 69.1, control 247 +/- 53.6, p < 0.01 in UIP versus NSIP or control); () the contractility positively correlated with the amount of either fibronectin (r = 0.867, p < 0.001, n = 15) or TGF-beta 1 (r = 0.939, p < 0.001, n = 15), respectively. Thus, UIP fibroblasts showed greater contractility than did NSIP fibroblasts and up-regulated control fibroblasts.
本研究旨在比较从特发性肺纤维化/普通间质性肺炎（UIP；n = 5）、非特异性间质性肺炎（NSIP；n = 5）患者的手术活检标本以及手术切除肺的正常部分（对照；n = 5）中获取的肺成纤维细胞的功能。结果显示：（1）与从NSIP或对照中获取的成纤维细胞相比，从UIP中获取的成纤维细胞收缩性增强（UIP为72.7 +/- 6.21%；NSIP为32.8 +/- 5.46；对照为28.5 +/- 3.51，UIP与NSIP或对照相比，p < 0.01）；（2）这种收缩性的增加与成纤维细胞中F - 肌动蛋白含量的增加一致；（3）UIP成纤维细胞培养的条件培养基增强了对照成纤维细胞的收缩性，而从NSIP或对照中获取的条件培养基则没有；（4）通过免疫印迹法，条件培养基中代表收缩性的180和25 kD产物分别被鉴定为纤连蛋白（ED - A结构域）和转化生长因子 - β1；（5）UIP条件培养基中纤连蛋白或转化生长因子 - β1的含量更高（纤连蛋白：UIP为289 +/- 47.1 ng/ml，NSIP为121 +/- 23.0，对照为118 +/- 16.0；转化生长因子 - β1：UIP为798 +/- 119 pg/ml，NSIP为246 +/- 69.1，对照为247 +/- 53.6，UIP与NSIP或对照相比p < 0.0；（6）收缩性分别与纤连蛋白量（r = 0.867，p < 0.001，n = 15）或转化生长因子 - β1量（r = 0.939，p < 0.001，n = 15）呈正相关。因此UIP成纤维细胞比NSIP成纤维细胞表现出更大的收缩性，并上调对照成纤维细胞的收缩性。
