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在体标记功能性核糖体揭示了嗜热栖热放线菌饥饿期间的空间调控。

In vivo labelling of functional ribosomes reveals spatial regulation during starvation in Podospora anserina.

作者信息

Lalucque H, Silar P

机构信息

Institut de Génétique et Microbiologie, Université de Paris-Sud, France.

出版信息

BMC Genet. 2000;1:3. doi: 10.1186/1471-2156-1-3. Epub 2000 Nov 16.

DOI:10.1186/1471-2156-1-3
PMID:11112985
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC29079/
Abstract

BACKGROUND

To date, in eukaryotes, ribosomal protein expression is known to be regulated at the transcriptional and/or translational levels. But other forms of regulation may be possible.

RESULTS

Here, we report the successful tagging of functional ribosomal particles with a S7-GFP chimaeric protein, making it possible to observe in vivo ribosome dynamics in the filamentous fungus Podospora anserina. Microscopic observations revealed a novel kind of ribosomal protein regulation during the passage between cell growth and stationary phases, with a transient accumulation of ribosomal proteins and/or ribosome subunits in the nucleus, possibly the nucleolus, being observed at the beginning of stationary phase.

CONCLUSION

Nuclear sequestration can be another level of ribosomal protein regulation in eukaryotic cells. This may contribute to the regulation of cell growth and division.

摘要

背景

迄今为止，在真核生物中，已知核糖体蛋白表达在转录和/或翻译水平受到调控。但其他形式的调控也有可能。

结果

在此，我们报告了用S7-GFP嵌合蛋白成功标记功能性核糖体颗粒，从而能够在丝状真菌嗜热栖热放线菌中观察核糖体的体内动态。显微镜观察揭示了细胞生长和静止期转换过程中一种新型的核糖体蛋白调控，在静止期开始时，观察到核糖体蛋白和/或核糖体亚基在细胞核（可能是核仁）中短暂积累。

结论

核隔离可能是真核细胞中核糖体蛋白调控的另一个层面。这可能有助于细胞生长和分裂的调控。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be37/29079/741a7f1ee573/1471-2156-1-3-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be37/29079/d939809e91b1/1471-2156-1-3-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be37/29079/741a7f1ee573/1471-2156-1-3-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be37/29079/d939809e91b1/1471-2156-1-3-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be37/29079/741a7f1ee573/1471-2156-1-3-2.jpg

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本文引用的文献

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The economics of ribosome biosynthesis in yeast.酵母中核糖体生物合成的经济学原理。
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