Follesa P, Cagetti E, Porta S, Espositoto G, Biggio G
Department of Experimental Biology, University of Cagliari, 09123 Cagliari, Italy.
Brain Res Mol Brain Res. 2000 Dec 8;84(1-2):52-7. doi: 10.1016/s0169-328x(00)00209-6.
The effect of treatment of rats with pivagabine (4-[(2,2-dimethyl-1-oxopropyl) amino] butanoic acid) for 4 days on the abundance of corticotropin-releasing factor (CRF) mRNA in the brain was investigated. Such treatment resulted in dose-dependent (100-300 mg/kg, i.p.) increases in the amount of CRF mRNA in both the hypothalamus and cerebral cortex. The maximal increases were thus apparent with the dose of 300 mg/kg in the hypothalamus (+108%) and cerebral cortex (+49%) 30 or 60 min, respectively, after the last drug injection. Foot-shock stress administered 30 min after the final drug injection had no effect on the pivagabine-induced increases in the abundance of CRF mRNA in the hypothalamus or cerebral cortex. Such stress also had no effect on the amounts of CRF mRNA in these brain regions of vehicle-treated rats. These results demonstrate that pivagabine increases the amount of CRF mRNA in both the hypothalamus and cerebral cortex of rats, effects that might be relevant to the action of this drug in preventing the stress-induced changes in CRF hypothalamic concentration.
研究了用吡戊加宾（4-[(2,2-二甲基-1-氧代丙基)氨基]丁酸）对大鼠进行4天治疗对其脑中促肾上腺皮质激素释放因子（CRF）mRNA丰度的影响。这种治疗导致下丘脑和大脑皮层中CRF mRNA量呈剂量依赖性（100 - 300 mg/kg，腹腔注射）增加。末次给药后30或60分钟，在下丘脑（+108%）和大脑皮层（+49%）中，300 mg/kg剂量时CRF mRNA量增加最为明显。末次给药后30分钟给予足部电击应激，对吡戊加宾诱导的下丘脑或大脑皮层中CRF mRNA丰度增加没有影响。这种应激对给予赋形剂处理大鼠的这些脑区中CRF mRNA量也没有影响。这些结果表明，吡戊加宾增加大鼠下丘脑和大脑皮层中CRF mRNA的量，这些作用可能与该药物预防应激诱导的下丘脑CRF浓度变化的作用有关。
