Ramasami S, Kerr K M, Chapman A D, King G, Cockburn J S, Jeffrey R R
Department of Pathology, Aberdeen University Medical School and Aberdeen Royal Infirmary, Foresterhill, Aberdeen, AB25 2ZB, UK.
J Pathol. 2000 Dec;192(4):427-32. doi: 10.1002/1096-9896(2000)9999:9999<::AID-PATH741>3.0.CO;2-Z.
Primary pulmonary adenocarcinoma was studied, looking for relationships between the expression of cell adhesion molecules (CAMs) E-cadherin, beta-catenin and CD44v6, and clinicopathological tumour parameters and patient post-operative survival. Formalin-fixed, paraffin-embedded tissue from 120 primary lung adenocarcinomas, including 23 poorly differentiated tumours, 17 of probable bronchial origin, and 29 with a prominent bronchioloalveolar pattern, together with nodal metastatic tumour from 34 of these patients was stained using monoclonal antibodies and immunohistochemistry. Sections were scored either high level (>10% cells positive) or low level (<10% positive). High level expression of CD44v6 was retained in 28.4% (34/120) of tumours, while high levels of E-cadherin (57.5%, 69/120) and beta-catenin (80. 8%, 97/120) were more frequent. For all CAMs, staining levels did not correlate with nodal status, stage or tumour type. The apical or basal staining seen in normal bronchial and alveolar epithelium was often seen in papillary, glandular, and bronchioloalveolar areas of tumour, while solid invasive tumour more often showed pericellular staining. When the staining for each CAM in 34 nodal metastases was compared with that in the corresponding primary tumour, a high degree of concordance was found, with no tendency for metastases to show less staining than the primary tumour. Expression of E-cadherin and beta-catenin in the primary tumour had no influence on post-operative survival, but patients whose tumours had low level CD44v6 expression had a poorer post-operative survival than those with high levels of CD44v6 (p=0.0014 for all patients, p=0.0012 for stage I patients only). In primary pulmonary adenocarcinoma, the levels of expression of E-cadherin, beta-catenin, and CD44v6 are not associated with lymph node metastases or tumour stage but the staining pattern is associated with tumour morphology. Low levels of CD44v6 expression predict a poor post-operative survival, independently of stage, while there is no such relationship with the expression of E-cadherin or beta-catenin.
对原发性肺腺癌进行研究，以探寻细胞黏附分子（CAMs）E-钙黏蛋白、β-连环蛋白和CD44v6的表达与临床病理肿瘤参数及患者术后生存率之间的关系。使用单克隆抗体和免疫组织化学方法，对120例原发性肺腺癌的福尔马林固定、石蜡包埋组织进行染色，其中包括23例低分化肿瘤、17例可能起源于支气管的肿瘤以及29例具有显著细支气管肺泡模式的肿瘤，同时对其中34例患者的淋巴结转移瘤进行染色。切片评分分为高水平（>10%细胞阳性）或低水平（<10%阳性）。28.4%（34/120）的肿瘤中CD44v6呈高水平表达，而E-钙黏蛋白（57.5%，69/120）和β-连环蛋白（80.8%，97/120）的高水平表达更为常见。对于所有CAMs，染色水平与淋巴结状态、分期或肿瘤类型均无相关性。在正常支气管和肺泡上皮中可见的顶端或基底染色，在肿瘤的乳头、腺泡和细支气管肺泡区域也常可见到，而实性浸润性肿瘤则更常表现为细胞周围染色。当比较34例淋巴结转移瘤中每种CAM的染色与相应原发性肿瘤的染色时，发现高度一致，转移瘤没有比原发性肿瘤染色更少的趋势。原发性肿瘤中E-钙黏蛋白和β-连环蛋白的表达对术后生存率没有影响，但肿瘤CD44v6表达水平低的患者术后生存率比CD44v6表达水平高的患者差（所有患者p=0.0014，仅I期患者p=0.0012）。在原发性肺腺癌中，E-钙黏蛋白、β-连环蛋白和CD44v6的表达水平与淋巴结转移或肿瘤分期无关，但染色模式与肿瘤形态有关。CD44v6低水平表达预示术后生存率差，与分期无关，而与E-钙黏蛋白或β-连环蛋白的表达无此关系。
