Moya J, Pizarro H, Jashés M, De Clercq E, Sandino A M
Facultad de Química y Biología, Laboratorio de Virología, Departamento de Ciencias Biológicas, Universidad de Santiago de Chile.
Antiviral Res. 2000 Nov;48(2):125-30. doi: 10.1016/s0166-3542(00)00122-4.
The in vivo antiviral effect of 5-ethynyl-1-beta-D-ribofuranosylimidazole-carboxamide (EICAR) was evaluated in coho salmon and rainbow trout fry, experimentally infected with infectious pancreatic necrosis virus (IPNV). Treatment consisted of a daily bath of 2 h in 0.4 microg ml(-1) or 0.8 microg ml(-1) of EICAR, for approximately 20 days. The behavior of the fish was studied for 45 days post-infection. The survival of the infected treated groups was compared with the survival of non-infected and infected untreated control groups. The results showed that the survival of coho salmon and rainbow trout fry in the infected group treated with both doses of EICAR was similar to the survival observed in the healthy control group (approximately 94%). While, the survival of the infected and untreated control fish was 56% for salmon and 28% for trout, there were no significant difference in the weight of coho salmon fry between those treated with EICAR and non-infected and infected untreated control groups. However, in rainbow trout there was a statistically significant weight decrease in infected untreated group. Finally, the analysis of tissue samples of the fish by reverse transcription associated with the polymerase chain reaction (RT-PCR) suggest that EICAR have decreased the viral load in infected treated fry. Consequently, the results indicate that EICAR is an effective inhibitor of IPNV replication in vivo and could be a promissory antiviral compound for the treatment of IPNV disease.
在感染传染性胰腺坏死病毒（IPNV）的银大麻哈鱼和虹鳟鱼苗中评估了5-乙炔基-1-β-D-呋喃核糖基咪唑-甲酰胺（EICAR）的体内抗病毒效果。治疗方法是将鱼每天在0.4微克/毫升或0.8微克/毫升的EICAR中浸泡2小时，持续约20天。在感染后45天对鱼的行为进行了研究。将感染处理组的存活率与未感染和感染未处理对照组的存活率进行了比较。结果表明，用两种剂量的EICAR处理的感染组中的银大麻哈鱼和虹鳟鱼苗的存活率与健康对照组中观察到的存活率相似（约94%）。而感染未处理的对照鱼的存活率，大麻哈鱼为56%，虹鳟为28%，用EICAR处理的银大麻哈鱼苗与未感染和感染未处理对照组之间的体重没有显著差异。然而，在虹鳟中，感染未处理组的体重有统计学上的显著下降。最后，通过与聚合酶链反应（RT-PCR）相关的逆转录对鱼的组织样本进行分析表明，EICAR降低了感染处理鱼苗中的病毒载量。因此，结果表明EICAR是IPNV体内复制的有效抑制剂，可能是治疗IPNV疾病的一种有前景的抗病毒化合物。
