Darbin O, Risso J J, Rostain J C
Laboratoire de Physiopathologie et Action Thérapeutique des Gaz Sous Pression, Université de la Méditerranée et IMNSSA, Institut J. Roche, Faculté de Médecine Nord, 13916 Cedex 20, Marseille, France.
Neurosci Lett. 2001 Jan 5;297(1):37-40. doi: 10.1016/s0304-3940(00)01654-2.
In rat, helium pressures induce locomotor and motor activity which requires dopaminergic and N-methyl-D-aspartate (NMDA) receptor activities at striatal level. However, biochemical studies have suggested that pressure exposure may increase striatal glutamate level. We used microdialysis technique to study the effects of pressure on glutamate level in the striatum and the effects of local administration of D1 (SCH23390) or D2 (sulpiride) on these changes. Pressures increase both glutamate and glutamine levels in striatal microdialysates. Administration of sulpiride (1 microM) or SCH23390 (1 microM) by reverse microdialysis did not affect significantly pressure induced glutamate increase. So, protective effects of D1 and D2 antagonists against locomotor and motor hyperactivity (LMA) are probably independent of the processes involved in the striatal glutamate increase evoked by pressure.
在大鼠中，氦气压力可诱导运动和运动活动，这需要纹状体水平的多巴胺能和N-甲基-D-天冬氨酸（NMDA）受体活性。然而，生化研究表明，压力暴露可能会增加纹状体谷氨酸水平。我们使用微透析技术来研究压力对纹状体中谷氨酸水平的影响，以及局部给予D1（SCH23390）或D2（舒必利）对这些变化的影响。压力会增加纹状体微透析液中的谷氨酸和谷氨酰胺水平。通过反向微透析给予舒必利（1 microM）或SCH23390（1 microM）对压力诱导的谷氨酸增加没有显著影响。因此，D1和D2拮抗剂对运动和运动亢进（LMA）的保护作用可能与压力引起的纹状体谷氨酸增加所涉及的过程无关。
