在晶体中捕获中间体：配体与肌红蛋白的结合

Trapping intermediates in the crystal: ligand binding to myoglobin.

作者信息

Schlichting I, Chu K

机构信息

Max Planck Institute for Molecular Physiology, Department for Physical Biochemistry, Otto-Hahn-Strasse 11, 44227, Dortmund, Germany.

出版信息

Curr Opin Struct Biol. 2000 Dec;10(6):744-52. doi: 10.1016/s0959-440x(00)00158-5.

DOI:10.1016/s0959-440x(00)00158-5

PMID:11114513

Abstract

Crystal structures of the reactive short-lived species that occur in chemical or binding reactions can be determined using X-ray crystallography via time-resolved or kinetic trapping approaches. Recently, various kinetic trapping methods have been used to determine the structure of intermediates in ligand binding to myoglobin.

摘要

通过时间分辨或动力学捕获方法，利用X射线晶体学可以确定在化学或结合反应中出现的反应性短寿命物种的晶体结构。最近，各种动力学捕获方法已被用于确定配体与肌红蛋白结合过程中中间体的结构。