Rajavarthini P B, Arunkumar R I, Michael R D
Centre for Fish Immunology, Postgraduate and Research Department of Zoology, American College, Madurai 625002.
Indian J Exp Biol. 2000 Jun;38(6):549-53.
Serum immunoglobulins of O. mossambicus were purified using chromatography methods--CM affinity gel blue chromatography followed by two step purification involving a combination of ion-exchange and gel filtration chromatography. Studies revealed that O. mossambicus produces only one class of high molecular weight macroglobulin as determined by molecular sieving by Sepharose CL 6-B. Immunoelectrophoresis of purified O. mossambicus serum against rabbit anti O. mossambicus serum gave only a single precipitin line. Further analysis of the immunoglobulin by SDS-PAGE showed that the IgM macroglobulin weighs about 900,000 Da, composed of mu-like heavy chain weighing about 90 kDa each and light chains weighing about 30 kDa each.
莫桑比克罗非鱼的血清免疫球蛋白采用色谱法进行纯化——先通过CM亲和凝胶蓝色谱法，然后进行两步纯化，这两步纯化涉及离子交换色谱法和凝胶过滤色谱法的组合。研究表明，通过Sepharose CL 6-B分子筛测定，莫桑比克罗非鱼仅产生一类高分子量巨球蛋白。用纯化的莫桑比克罗非鱼血清对兔抗莫桑比克罗非鱼血清进行免疫电泳，仅产生一条沉淀线。通过SDS-PAGE对免疫球蛋白进行进一步分析表明，IgM巨球蛋白的重量约为900,000 Da，由每条重约90 kDa的μ样重链和每条重约30 kDa的轻链组成。
