Talesara C L, Vashishta N
Muscle Physiology & Histochemistry Unit, Department of Zoology, University of Delhi, Delhi 110007, India.
Indian J Exp Biol. 2000 Jun;38(6):567-74.
The present study analyzes the regeneration of skeletal muscle in streptozotocin (STZ) induced diabetic rats. Two weeks later the gastrocnemius muscle from diabetic rats were transplanted into diabetic and normal host to initiate regeneration and the normal gastrocnemius muscle was transplanted in normal and diabetic hosts for comparison. The regenerates were analyzed after 15 and 30 days of transplantation for histochemical (with respect to SDH and m-ATPase) and 7, 14, 21 and 28 days after transplantation for biochemical studies (with respect to SDH and m-ATPase). Least enzymatic activity and the poorest regenerative ability in case of normal muscle in diabetic host (NM-DbH) and comparatively higher enzymatic activity and better regenerative ability in diabetic muscle in normal host (DbM-NH) was observed. The result of this study strongly supports that the normal host environment is crucial for the muscle recovery.
本研究分析了链脲佐菌素（STZ）诱导的糖尿病大鼠骨骼肌的再生情况。两周后，将糖尿病大鼠的腓肠肌移植到糖尿病和正常宿主中以启动再生，并将正常腓肠肌移植到正常和糖尿病宿主中进行比较。移植15天和30天后对再生组织进行组织化学分析（针对琥珀酸脱氢酶（SDH）和肌型ATP酶（m-ATPase）），移植7天、14天、21天和28天后进行生化研究（针对SDH和m-ATPase）。观察到糖尿病宿主中的正常肌肉（NM-DbH）酶活性最低且再生能力最差，而正常宿主中的糖尿病肌肉（DbM-NH）酶活性相对较高且再生能力较好。本研究结果有力地支持了正常宿主环境对肌肉恢复至关重要这一观点。
