Vanifatova N G, Spivakov B Y, Mattusch J, Wennrich R
Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Moscow.
J Chromatogr A. 2000 Nov 17;898(2):257-63. doi: 10.1016/s0021-9673(00)00850-5.
Size separation of five unmodified polystyrene nanosphere standards with diameters between 50 and 600 nm has been achieved in phosphate buffer solutions as carrier electrolyte. The electrophoretic mobility increases with particle diameter. Optical spectra was shown to be different for particles of different size. Effects of injection time, applied voltage, pH, and phosphate concentration in carrier electrolyte on particle separation were studied. Under optimal conditions the peak efficiency ranged from 600 to 10,500 theoretical plate numbers depending on nanosphere diameter was achieved.
以磷酸盐缓冲溶液作为载流电解质，实现了直径在50至600纳米之间的五种未改性聚苯乙烯纳米球标准品的尺寸分离。电泳迁移率随粒径增大而增加。不同尺寸的颗粒显示出不同的光谱。研究了进样时间、施加电压、pH值和载流电解质中磷酸盐浓度对颗粒分离的影响。在最佳条件下，根据纳米球直径的不同，峰效率范围为600至10500理论塔板数。
