小肠癌中基因改变与细胞增殖的联合分析

Combination analysis of genetic alterations and cell proliferation in small intestinal carcinomas.

作者信息

Muneyuki T, Watanabe M, Yamanaka M, Isaji S, Kawarada Y, Yatani R

机构信息

First Department of Surgery, Mie University School of Medicine, Tsu-shi, Japan.

出版信息

Dig Dis Sci. 2000 Oct;45(10):2022-8. doi: 10.1023/a:1005560428937.

DOI:10.1023/a:1005560428937

PMID:11117578

Abstract

We analyzed K-ras and p53 mutations, microsatellite instability (MSI), and loss of heterozygosity (LOH) using the polymerase chain reaction (PCR) methods, as well as p53 expression and the Ki-67 labeling index (LI) using immunohistochemistry in 10 duodenal and 10 jejunal/ileal carcinomas. K-ras mutations were detected in two duodenal (20%) and three jejunal/ileal (30%) carcinomas and p53 mutations in one (10%) and three (30%), respectively. LOH of 17p was detected in two duodenal (20%) and two jejunal/ileal (20%) carcinomas and p53 expression in four duodenal (40%) and four jejunal/ileal (40%). One duodenal (10%) and two jejunal/ileal (20%) carcinomas demonstrated MSI. LOH of APC was detected in three jejunal/ileal (30%), but none of the duodenal carcinomas. The Ki-67 LI was 44% in duodenal and 52.6% in jejunal/ileal carcinomas. A subset of small intestinal carcinomas showed involvement of K-ras and p53 mutations, LOH of APC, and MSI. A difference was also apparent for LOH of APC between duodenal and jejunal/ileal carcinomas.

摘要

我们采用聚合酶链反应（PCR）方法分析了10例十二指肠癌和10例空肠/回肠癌中的K-ras和p53突变、微卫星不稳定性（MSI）以及杂合性缺失（LOH），并通过免疫组织化学检测了p53表达和Ki-67标记指数（LI）。在2例十二指肠癌（20%）和3例空肠/回肠癌（30%）中检测到K-ras突变，在1例（10%）十二指肠癌和3例（30%）空肠/回肠癌中检测到p53突变。在2例十二指肠癌（20%）和2例空肠/回肠癌（20%）中检测到17p的LOH，在4例十二指肠癌（40%）和4例空肠/回肠癌（40%）中检测到p53表达。有1例十二指肠癌（10%）和2例空肠/回肠癌（20%）表现为MSI。在3例空肠/回肠癌（30%）中检测到APC的LOH，但十二指肠癌中均未检测到。十二指肠癌的Ki-67 LI为44%，空肠/回肠癌为52.6%。一部分小肠癌显示出K-ras和p53突变、APC的LOH以及MSI。十二指肠癌和空肠/回肠癌之间在APC的LOH方面也存在明显差异。