赤霉素生物合成：水稻早期13-羟基化途径中三个步骤的代谢证据

Gibberellin biosynthesis: metabolic evidence for three steps in the early 13-hydroxylation pathway of rice.

作者信息

Kobayashi M, MacMillan J, Phinney B, Gaskin P, Spray C R, Hedden P

机构信息

Plant Molecular Biology Laboratory, Tsukuba Life Science Center, The Institute of Chemical and Physical Research, Tsukuba-Ibaraki, Japan.

出版信息

Phytochemistry. 2000 Oct;55(4):317-21. doi: 10.1016/s0031-9422(00)00265-x.

DOI:10.1016/s0031-9422(00)00265-x

PMID:11117879

Abstract

[14C4]GA53, [14C4]GA44, and [2H2/14C4]GA19 were injected separately into seedlings of rice (Oryza sativa) using a dwarf mutant (d35) that has low levels of endogenous gibberellins (GAs). After 8 h incubation, the shoots were extracted and the labeled metabolites were identified by full-scan gas chromatography mass spectrometry (GC-MS) and Kovats retention indices (KRIs). Our results document the metabolic sequence, GA53-->GA44-->GA19-->GA20 and the presence of endogenous GA53, GA44, GA19, GA20 and GA1. Previous metabolic studies have shown the presence of the step, GA20-->GA1 in rice. Taken together, the data establish in vegetative shoots of rice the presence of the early 13-hydroxylation pathway, a pathway that originates from GA12 and leads to bioactive GA1.

摘要

利用内源赤霉素（GA）水平较低的矮化突变体（d35），将[14C4]GA53、[14C4]GA44和[2H2/14C4]GA19分别注射到水稻（Oryza sativa）幼苗中。孵育8小时后，提取地上部分，并通过全扫描气相色谱 - 质谱联用仪（GC-MS）和科瓦茨保留指数（KRIs）鉴定标记代谢物。我们的结果记录了代谢序列，GA53→GA44→GA19→GA20以及内源GA53、GA44、GA19、GA20和GA1的存在。先前的代谢研究表明水稻中存在GA20→GA1这一步骤。综合来看，这些数据证实了在水稻营养地上部分存在早期13-羟基化途径，该途径起源于GA12并导致生物活性GA1的产生。