Oh S H, Miyazaki M, Kouchi H, Inoue Y, Sakaguchi M, Tsuji T, Shima N, Higashio K, Namba M
Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan.
Biochem Biophys Res Commun. 2000 Dec 20;279(2):500-4. doi: 10.1006/bbrc.2000.3985.
Bone marrow (BM) cells originally include alpha-fetoprotein (AFP)- and c-Met [a receptor for hepatocyte growth factor (HGF)]-expressing cells. In vitro treatment of BM cells with HGF induced albumin-expressing hepatocyte-like cells. Furthermore, those hepatocyte-like cells expressed cytokeratins 8 and 18, which are typically expressed in normal adult hepatocytes. These findings demonstrate that BM cells include AFP-expressing hepatic progenitor cells that can be differentiated into hepatocytes by HGF in culture, indicating that such cultures are useful resources for cell transplantation therapy for liver diseases.
骨髓（BM）细胞最初包括表达甲胎蛋白（AFP）和c-Met [肝细胞生长因子（HGF）的一种受体]的细胞。用HGF对BM细胞进行体外处理可诱导产生表达白蛋白的肝细胞样细胞。此外，那些肝细胞样细胞表达细胞角蛋白8和18，这两种蛋白通常在正常成年肝细胞中表达。这些发现表明，BM细胞包括表达AFP的肝祖细胞，其在培养中可被HGF分化为肝细胞，这表明此类培养物是用于肝病细胞移植治疗的有用资源。
