Carrillo A, Rising R, Cole C, Tverskaya R, Lifshitz F
Miami Children's Hospital, Research Institute, Miami, Florida 33155, USA.
Nutrition. 2000 Nov-Dec;16(11-12):1074-8. doi: 10.1016/s0899-9007(00)00435-4.
In a previous study, weight gain, insulin growth factor-1 (IGF-1) and insulin growth factor binding protein-3 (IGFBP-3) were increased in rats fed suboptimal levels of energy and administered 0.1 mg/100 g of body weight of recombinant human growth hormone (rhGH). Our objective was to determine whether these anabolic effects were still obtained with lower dosages of rhGH in similarly fed rats. Three groups of male, prepubertal Sprague-Dawley rats were administered rhGH and three groups of similar rats were given normal saline solution daily (0.05 mg/100 g of body weight subcutaneously). All rats were fed a balanced 1:1 carbohydrate:fat ratio diet for 4 wk. Restricted rats within each treatment were pair fed 80% and 60% ad libitum. Daily body weight, food intake, and efficiency were recorded. After 4 wk, serum IGF-1 and IGFBP-3, body fat, fat-free mass, and total body water were determined. Total weight gain and serum IGFBP-3 were significantly higher, with a tendency for increased body fat, in rats treated with rhGH and fed at 60% ad libitum. However, within each treatment, energy restriction caused decreased body fat and total body water. These results suggest that lower dosages of rhGH provide anabolic effects during suboptimal energy intake.
在先前的一项研究中，给能量摄入不足且皮下注射0.1毫克/100克体重重组人生长激素（rhGH）的大鼠喂食后，其体重增加、胰岛素样生长因子-1（IGF-1）和胰岛素样生长因子结合蛋白-3（IGFBP-3）均有所增加。我们的目的是确定在同样喂食的大鼠中，较低剂量的rhGH是否仍能产生这些合成代谢效应。将三组青春期前雄性斯普拉格-道利大鼠给予rhGH，另外三组相似的大鼠每天给予生理盐水溶液（皮下注射0.05毫克/100克体重）。所有大鼠均喂食碳水化合物与脂肪比例为1:1的均衡饮食4周。每种处理中受限制的大鼠按随意摄入量的80%和60%进行配对喂食。记录每日体重、食物摄入量和效率。4周后，测定血清IGF-1和IGFBP-3、体脂、去脂体重和全身水含量。在以随意摄入量的60%喂食并接受rhGH治疗的大鼠中，总体重增加和血清IGFBP-3显著更高，且有体脂增加的趋势。然而，在每种处理中，能量限制导致体脂和全身水含量降低。这些结果表明，在能量摄入不足期间，较低剂量的rhGH可产生合成代谢效应。
