Ishikawa Y, Akasaka Y, Ishii T, Itoh K, Masuda T, Zhang L, Kiguchi H
Department of Pathology, Toho University School of Medicine, Tokyo, Japan.
Histopathology. 2000 Dec;37(6):546-54. doi: 10.1046/j.1365-2559.2000.00994.x.
The myocardium expresses vascular endothelial growth factor (VEGF), heat shock protein 70 (HSP70), and ubiquitin immediately after the onset of cardiac ischaemia. This study demonstrated the sequential changes in localization of these proteins, in addition to fibronectin and troponin T (TnT), in human hearts with myocardial infarction (MI).
Myocardial tissues from 40 autopsied MI cases were immunostained with the five antibodies against VEGF, HSP70, ubiquitin, fibronectin and TnT. Fibronectin was recognized only in the cardiomyocytes with infarction. Although TnT, HSP70, ubiquitin and VEGF were detected in the affected myocardium in the early stages, their expression in cardiomyocytes around infarcted foci were more intense. The cardiomyocytes with coagulative myocytolysis were positive for fibronectin, but negative or weakly positive for TnT, HSP70, ubiquitin and VEGF. In contrast, the cardiomyocytes with colliquative myocytolysis were strongly positive for TnT, HSP70, ubiquitin and VEGF, but negative for fibronectin.
Immunostaining using antibodies to fibronectin, TnT, HSP70, ubiquitin and VEGF is useful for the discrimination between infarcted myocytes and ischaemia-damaged myocytes in the human heart with MI at autopsy.
心肌在心脏缺血发作后立即表达血管内皮生长因子（VEGF）、热休克蛋白70（HSP70）和泛素。本研究展示了在心肌梗死（MI）的人体心脏中，除纤连蛋白和肌钙蛋白T（TnT）外，这些蛋白质定位的顺序变化。
用针对VEGF、HSP70、泛素、纤连蛋白和TnT的五种抗体对40例MI尸检病例的心肌组织进行免疫染色。仅在梗死的心肌细胞中识别出纤连蛋白。虽然在早期受影响的心肌中检测到TnT、HSP70、泛素和VEGF，但它们在梗死灶周围心肌细胞中的表达更强。发生凝固性肌溶解的心肌细胞对纤连蛋白呈阳性，但对TnT、HSP70、泛素和VEGF呈阴性或弱阳性。相反，发生液化性肌溶解的心肌细胞对TnT、HSP70、泛素和VEGF呈强阳性，但对纤连蛋白呈阴性。
使用针对纤连蛋白、TnT、HSP70、泛素和VEGF的抗体进行免疫染色，有助于在尸检时鉴别MI人体心脏中的梗死心肌细胞和缺血损伤心肌细胞。
