Marion-Latard F, De Glisezinski I, Crampes F, Berlan M, Galitzky J, Suljkovicova H, Riviere D, Stich V
Laboratory of the Adaptations to Exercise, Purpan University Hospital, 31059 Toulouse Cedex, France.
Am J Physiol Regul Integr Comp Physiol. 2001 Jan;280(1):R166-73. doi: 10.1152/ajpregu.2001.280.1.R166.
This study was designed to assess whether physiological activation of the sympathetic nervous system induced by exercise changes adipose tissue responsiveness to catecholamines in humans. Lipid mobilization in abdominal subcutaneous adipose tissue was studied with the use of a microdialysis method in 11 nontrained men (age: 22. 3 +/- 1.5 yr; body mass index: 23.0 +/- 1.6). Adipose tissue adrenergic sensitivity was explored with norepinephrine, dobutamine (beta(1)-agonist), or terbutaline (beta(2)-agonist) perfused during 30 min through probes before and after 60-min exercise (50% of the maximal aerobic power). The increase in extracellular glycerol concentration during infusion was significantly lower after the exercise when compared with the increase observed before the exercise (P < 0.05, P < 0.02, and P < 0.01, respectively, for norepinephrine, dobutamine, and terbutaline). In a control experiment realized without exercise, no difference in norepinephrine-induced glycerol increase between the two infusions was observed. To assess the involvement of catecholamines in the blunted beta-adrenergic-induced lipolytic response after exercise, adipose tissue adrenergic sensitivity was explored with two 60-min infusions of norepinephrine or epinephrine separated by a 60-min interval. With both catecholamines, the increase in glycerol was significantly lower during the second infusion (P < 0.05). The findings suggest that aerobic exercise, which increased adrenergic activity, induces a desensitization in beta(1)- and beta(2)-adrenergic lipolytic pathways in human subcutaneous adipose tissue.
本研究旨在评估运动诱导的交感神经系统生理性激活是否会改变人体脂肪组织对儿茶酚胺的反应性。采用微透析法对11名未经训练的男性（年龄：22.3±1.5岁；体重指数：23.0±1.6）的腹部皮下脂肪组织中的脂质动员情况进行了研究。在60分钟运动（最大有氧功率的50%）前后，通过探针灌注去甲肾上腺素、多巴酚丁胺（β1激动剂）或特布他林（β2激动剂）30分钟，以探究脂肪组织的肾上腺素能敏感性。与运动前观察到的增加相比，运动后输注期间细胞外甘油浓度的增加显著降低（去甲肾上腺素、多巴酚丁胺和特布他林分别为P<0.05、P<0.02和P<0.01）。在无运动的对照实验中，两次输注之间去甲肾上腺素诱导的甘油增加没有差异。为了评估儿茶酚胺在运动后β肾上腺素能诱导的脂肪分解反应减弱中的作用，通过间隔60分钟的两次60分钟去甲肾上腺素或肾上腺素输注来探究脂肪组织的肾上腺素能敏感性。使用这两种儿茶酚胺时，第二次输注期间甘油的增加显著降低（P<0.05）。研究结果表明，增加肾上腺素能活性的有氧运动可导致人体皮下脂肪组织中β1和β2肾上腺素能脂肪分解途径的脱敏。
